PUBLICATION
Integrated molecular analysis identifies a conserved pericyte gene signature in zebrafish
- Authors
- Shih, Y.H., Portman, D., Idrizi, F., Grosse, A., Lawson, N.D.
- ID
- ZDB-PUB-211110-7
- Date
- 2021
- Source
- Development (Cambridge, England) 148(23): (Journal)
- Registered Authors
- Lawson, Nathan, Shih, Yu-huan
- Keywords
- Pericyte, Vascular development, Zebrafish
- Datasets
- GEO:GSE176129
- MeSH Terms
-
- Animals
- Electron Transport Complex IV/biosynthesis
- Electron Transport Complex IV/genetics
- Gene Expression Regulation, Developmental*
- Muscle, Smooth, Vascular/metabolism*
- Mutation
- Myocytes, Smooth Muscle/metabolism*
- Pericytes/metabolism*
- Receptor, Platelet-Derived Growth Factor beta/biosynthesis
- Receptor, Platelet-Derived Growth Factor beta/genetics
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/biosynthesis*
- Zebrafish Proteins/genetics
- PubMed
- 34751773 Full text @ Development
Citation
Shih, Y.H., Portman, D., Idrizi, F., Grosse, A., Lawson, N.D. (2021) Integrated molecular analysis identifies a conserved pericyte gene signature in zebrafish. Development (Cambridge, England). 148(23):.
Abstract
Pericytes reside in capillary beds where they share a basement membrane with endothelial cells and regulate their function. However, little is known about embryonic pericyte development, in part, due to lack of specific molecular markers and genetic tools. Here, we applied single cell RNA-sequencing (scRNA-seq) of platelet derived growth factor beta (pdgfrb)-positive cells to molecularly characterize pericytes in zebrafish larvae. scRNA-seq revealed zebrafish cells expressing mouse pericyte gene orthologs while comparison to bulk RNA-seq from wild type and pdgfrb mutant larvae further refined a pericyte geneset. Subsequent integration with mouse pericyte scRNA-seq profiles revealed a core set of conserved pericyte genes. Using transgenic reporter lines, we validated pericyte expression of two genes identified in our analysis: NDUFA4 mitochondrial complex associated like 2a (ndufa4l2a), and potassium voltage-gated channel, Isk-related family, member 4 (kcne4). Both reporter lines exhibited pericyte expression in multiple anatomical locations, while kcne4 was also detected in a subset of vascular smooth muscle cells. Thus, our integrated molecular analysis revealed a molecular profile for zebrafish pericytes and allowed us to develop new tools to observe these cells in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping