PUBLICATION
Is catalase an effective additive to alleviate oxidative stress during cryopreservation of zebrafish sperm at the repository level?
- Authors
- Yang, H., Hu, E., Matthews, J.L., Varga, Z.M., Tiersch, T.R.
- ID
- ZDB-PUB-211104-2
- Date
- 2021
- Source
- Cryobiology 104: 70-78 (Journal)
- Registered Authors
- Matthews, Jennifer, Varga, Zoltán M.
- Keywords
- Cryoprotectant, Danio rerio, Decision-making, In vitro fertilization, Motility, Plasma membrane integrity, Process-optimization, Sperm cell survival
- MeSH Terms
-
- Animals
- Catalase/metabolism
- Cryopreservation*/methods
- Cryoprotective Agents/metabolism
- Cryoprotective Agents/pharmacology
- Male
- Methanol/pharmacology
- Oxidative Stress
- Semen Preservation*/veterinary
- Sperm Motility
- Spermatozoa
- Zebrafish
- PubMed
- 34728226 Full text @ Cryobiology
Citation
Yang, H., Hu, E., Matthews, J.L., Varga, Z.M., Tiersch, T.R. (2021) Is catalase an effective additive to alleviate oxidative stress during cryopreservation of zebrafish sperm at the repository level?. Cryobiology. 104:70-78.
Abstract
The goal of this study was to investigate whether supplementation of cryoprotective medium with catalase (CAT), an antioxidation enzyme, is efficient for zebrafish sperm cryopreservation from the viewpoint of high-throughput genetic repository operations. Three cryoprotectants (10%, v/v), dimethylacetamide (DMA), dimethylformamide (DMF), and methanol were used. The objectives were to evaluate the effects of CAT on sperm motility, plasma membrane integrity, and concentration: 1) fresh sperm at equilibration up to 60 min; 2) post-thaw sperm after cooling at 10, 20, and 40 °C/min), and 3) post-thaw fertilization and embryo survival rates. Catalase addition did not improve sperm motility, regardless of the cryoprotectants added. After 10-min exposure to DMA and methanol, membrane integrity was significantly decreased (70-75%) compared to controls. With catalase, sperm cells were maintained membrane integrity and after 50 min equilibration, cell concentrations were maintained with CAT compared to cryoprotectant-only test groups. However, after cryopreservation and thawing, CAT did not affect the outcome of motility, membrane integrity, cell concentration, fertilization, or embryo survival assays. Analysis of cooling rates also indicated that CAT did not affect 3-hpf fertilization or 24-hpf survival rates. Overall, addition of CAT could provide some protection of sperm from oxidative stress before freezing, but not after thawing. We propose that decisions concerning routine use of CAT for repositories, especially those handling tens of thousands of frozen samples per year, would depend on whether efficient high-throughput operation, or specific research questions are programmatic goals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping