PUBLICATION

Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle

Authors
Lo, H.P., Lim, Y.W., Xiong, Z., Martel, N., Ferguson, C., Ariotti, N., Giacomotto, J., Rae, J., Floetenmeyer, M., Moradi, S.V., Gao, Y., Tillu, V.A., Xia, D., Wang, H., Rahnama, S., Nixon, S.J., Bastiani, M., Day, R.D., Smith, K.A., Palpant, N.J., Johnston, W.A., Alexandrov, K., Collins, B.M., Hall, T.E., Parton, R.G.
ID
ZDB-PUB-211012-14
Date
2021
Source
The Journal of cell biology   220(12): (Journal)
Registered Authors
Giacomotto, Jean, Hall, Thomas, Lo, Harriet, Parton, Robert G., Smith, Kelly
Keywords
none
MeSH Terms
  • Adaptor Proteins, Signal Transducing/metabolism*
  • Animals
  • Caveolae/metabolism
  • Cell Line
  • Embryo, Nonmammalian/metabolism
  • Imaging, Three-Dimensional
  • Mice, Inbred C57BL
  • Muscle Fibers, Skeletal/metabolism
  • Muscle Fibers, Skeletal/ultrastructure
  • Muscle Proteins/metabolism*
  • Muscle, Skeletal/metabolism*
  • Muscle, Skeletal/ultrastructure
  • Nerve Tissue Proteins/metabolism*
  • Protein Binding
  • Sarcolemma/metabolism*
  • Sarcolemma/ultrastructure
  • Tumor Suppressor Proteins/metabolism*
  • Zebrafish/embryology
  • Zebrafish/metabolism*
  • Zebrafish Proteins/metabolism*
PubMed
34633413 Full text @ J. Cell Biol.
Abstract
The cavin proteins are essential for caveola biogenesis and function. Here, we identify a role for the muscle-specific component, Cavin4, in skeletal muscle T-tubule development by analyzing two vertebrate systems, mouse and zebrafish. In both models, Cavin4 localized to T-tubules, and loss of Cavin4 resulted in aberrant T-tubule maturation. In zebrafish, which possess duplicated cavin4 paralogs, Cavin4b was shown to directly interact with the T-tubule-associated BAR domain protein Bin1. Loss of both Cavin4a and Cavin4b caused aberrant accumulation of interconnected caveolae within the T-tubules, a fragmented T-tubule network enriched in Caveolin-3, and an impaired Ca2+ response upon mechanical stimulation. We propose a role for Cavin4 in remodeling the T-tubule membrane early in development by recycling caveolar components from the T-tubule to the sarcolemma. This generates a stable T-tubule domain lacking caveolae that is essential for T-tubule function.
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