PUBLICATION
3DM: deep decomposition and deconvolution microscopy for rapid neural activity imaging
- Authors
- Cho, E.S., Han, S., Lee, K.H., Kim, C.H., Yoon, Y.G.
- ID
- ZDB-PUB-211008-1
- Date
- 2021
- Source
- Optics express 29: 32700-32711 (Journal)
- Registered Authors
- Kim, Cheol-Hee
- Keywords
- none
- MeSH Terms
-
- Animals
- Brain/diagnostic imaging*
- Brain/physiology
- Imaging, Three-Dimensional/methods*
- Intravital Microscopy/methods
- Larva
- Microscopy, Confocal
- Microscopy, Fluorescence/methods*
- Neural Networks, Computer
- Neurons/physiology
- Optical Imaging/methods*
- Spinal Cord/diagnostic imaging
- Spinal Cord/physiology
- Zebrafish/physiology*
- PubMed
- 34615335 Full text @ Opt. Express
Citation
Cho, E.S., Han, S., Lee, K.H., Kim, C.H., Yoon, Y.G. (2021) 3DM: deep decomposition and deconvolution microscopy for rapid neural activity imaging. Optics express. 29:32700-32711.
Abstract
We report the development of deep decomposition and deconvolution microscopy (3DM), a computational microscopy method for the volumetric imaging of neural activity. 3DM overcomes the major challenge of deconvolution microscopy, the ill-posed inverse problem. We take advantage of the temporal sparsity of neural activity to reformulate and solve the inverse problem using two neural networks which perform sparse decomposition and deconvolution. We demonstrate the capability of 3DM via in vivo imaging of the neural activity of a whole larval zebrafish brain with a field of view of 1040 µm × 400 µm × 235 µm and with estimated lateral and axial resolutions of 1.7 µm and 5.4 µm, respectively, at imaging rates of up to 4.2 volumes per second.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping