PUBLICATION

Integration of multiple imaging platforms to uncover cardiovascular defects in adult zebrafish

Authors

Bensimon-Brito, A., Boezio, G.L.M., Cardeira-da-Silva, J., Wietelmann, A., Ramkumar, S., Lundegaard, P.R., Helker, C.S.M., Ramadass, R., Piesker, J., Nauerth, A., Mueller, C., Stainier, D.Y.R.

ID

ZDB-PUB-211006-6

Date

2021

Source

Cardiovascular research 118(12): 2665-2687 (Journal)

Registered Authors

Bensimon-Brito, Anabela, Helker, Christian, Lundegaard, Pia Rengtved, Stainier, Didier

Keywords

none

MeSH Terms

Animals
Cardiovascular System*
Mammals
Echocardiography
X-Ray Microtomography
Humans
Zebrafish*/genetics
Heart

(all 8)

PubMed

34609500 Full text @ Cardiovasc. Res.

Abstract

Aims Mammalian models have been instrumental in investigating adult heart function and human disease. However, electrophysiological differences with human hearts and high costs motivate the need for non-mammalian models. The zebrafish is a well-established genetic model to study cardiovascular development and function; however, analysis of cardiovascular phenotypes in adult specimens is particularly challenging as they are opaque.

Methods and results Here, we optimized and combined multiple imaging techniques including echocardiography, magnetic resonance imaging, and micro-computed tomography to identify and analyze cardiovascular phenotypes in adult zebrafish. Using alk5a/tgfbr1a mutants as a case study, we observed morphological and functional cardiovascular defects that were undetected with conventional approaches. Correlation analysis of multiple parameters revealed an association between hemodynamic defects and structural alterations of the heart, as observed clinically.

Conclusion We report a new, comprehensive, and sensitive platform to identify otherwise indiscernible cardiovascular phenotypes in adult zebrafish.

Translational perspective This study further illustrates the importance of the zebrafish model to investigate cardiovascular phenotypes including morphological and functional alterations as observed in human disease settings.

Genes / Markers

Figures

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Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
tgfbr1a^{bns329/bns329}; s843Tg	standard conditions	Adult	blood circulation process quality, abnormal	Fig. 6
tgfbr1a^{bns329/bns329}; s843Tg	standard conditions	Adult	blood circulation process quality, abnormal	Fig. 2
tgfbr1a^{bns329/bns329}; s843Tg	standard conditions	Adult	bulbus arteriosus ab1-elnb labeling decreased amount, abnormal	Fig. 1
tgfbr1a^{bns329/bns329}; s843Tg	standard conditions	Adult	bulbus arteriosus anatomical space dilated, abnormal	Fig. 3
tgfbr1a^{bns329/bns329}; s843Tg	standard conditions	Adult	bulbus arteriosus anatomical space dilated, abnormal	Fig. 1
tgfbr1a^{bns329/bns329}; s843Tg	standard conditions	Adult	bulbus arteriosus lumen dilated, abnormal	Fig. 6
tgfbr1a^{bns329/bns329}; s843Tg	standard conditions	Adult	bulbus arteriosus lumen dilated, abnormal	Fig. 4
tgfbr1a^{bns329/bns329}; s843Tg	standard conditions	Adult	dorsal aorta blood increased fluid flow, abnormal	Fig. 2
tgfbr1a^{bns329/bns329}; s843Tg	standard conditions	Adult	whole organism length, normal	Fig. 1
tgfbr1a^{bns329/bns329}; s843Tg	standard conditions	Adult	whole organism volume, normal	Fig. 1

1 - 10 of 11

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
bns329		Indel	tgfbr1a
s843Tg	Tg(kdrl:EGFP)	Transgenic Insertion

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
s843Tg
tgfbr1a^{bns329/bns329}; s843Tg

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP

Mapping

Bensimon-Brito et al., 2021 ZDB-PUB-211006-6 PMID:34609500

Integration of multiple imaging platforms to uncover cardiovascular defects in adult zebrafish

Bensimon-Brito et al., 2021

ZDB-PUB-211006-6
PMID:34609500