PUBLICATION
Structure features, selenylation modification, and improved anti-tumor activity of a polysaccharide from Eriobotrya japonica
- Authors
- Zhang, S., Zhang, H., Shi, L., Li, Y., Tuerhong, M., Abudukeremu, M., Cui, J., Li, Y., Jin, D.Q., Xu, J., Guo, Y.
- ID
- ZDB-PUB-210926-1
- Date
- 2021
- Source
- Carbohydrate Polymers 273: 118496 (Journal)
- Registered Authors
- Cui, Jianlin, Li, Yuhao
- Keywords
- Anti-tumor activity, Eriobotrya japonica, Polysaccharide, Selenylation, Zebrafish xenograft
- MeSH Terms
-
- A549 Cells
- Animals
- Antineoplastic Agents/pharmacology*
- Apoptosis/drug effects
- Cell Proliferation/drug effects
- Eriobotrya/chemistry*
- Hep G2 Cells
- Humans
- Neoplasms/drug therapy*
- Neoplasms/metabolism
- Neovascularization, Pathologic/metabolism
- Nitric Acid/chemistry
- Photoelectron Spectroscopy/methods
- Plant Leaves/chemistry
- Polysaccharides/chemistry
- Polysaccharides/pharmacology*
- Selenium/chemistry*
- Zebrafish
- PubMed
- 34560937 Full text @ Carbohydr. Polym.
Citation
Zhang, S., Zhang, H., Shi, L., Li, Y., Tuerhong, M., Abudukeremu, M., Cui, J., Li, Y., Jin, D.Q., Xu, J., Guo, Y. (2021) Structure features, selenylation modification, and improved anti-tumor activity of a polysaccharide from Eriobotrya japonica. Carbohydrate Polymers. 273:118496.
Abstract
A homogeneous polysaccharide, EJP90-1, was isolated from the leaves of E. japonica by hot water extraction in this study. EJP90-1 (7702 Da) was a heteropolysaccharide mainly consisting of →5)-linked-α-L-Araf-(1→, →4)-linked-β-D-Manp-(1→, →2,4)-linked-α-L-Rhap-(1→, →4)-linked-α-D-Xylp-(1→, →4)-linked-β-D-Galp-(1→, →2)-linked-β-D-Galp-(1→, →6)-linked-β-D-Glcp-(1→, α-D-Glcp-(4→, and t-linked-α-L-Araf. EJP90-1 was found to show moderate anti-tumor activity at the cellular level. In order to improve the anti-tumor activity and the potential applications of EJP90-1, a typical sodium selenite-nitric acid (Na2SeO3-HNO3) modification on EJP90-1 was carried out. X-ray photoelectron spectroscopy (XPS) and energy dispersive spectrometer (EDS) analysis confirmed that Se was successfully introduced into the polymer chain of EJP90-1. The subsequent in vitro cytotoxicity evaluation showed the selenylation modification derivative (EJP90-1-Se) possessed significant antiproliferative activity against cancer cells (HepG2 and A549 cells) through inducing cell apoptosis. The anti-tumor activity of EJP90-1-Se was further confirmed by zebrafish models, which inhibited the proliferation and migration of HepG2 cells and the angiogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping