PUBLICATION
A rapid and simple procedure for the isolation and cultivation of fibroblast-like cells from medaka and zebrafish embryos and fin clip biopsies
- Authors
- Beedgen, L., Hüllen, A., Gücüm, S., Thumberger, T., Wittbrodt, J., Thiel, C.
- ID
- ZDB-PUB-210924-1
- Date
- 2021
- Source
- Laboratory animals 56(3): 270-278 (Journal)
- Registered Authors
- Wittbrodt, Jochen
- Keywords
- Fibroblast-like cells, embryo, fibroblasts, fin clip, fish cell culture, medaka, zebrafish
- MeSH Terms
-
- Animals
- Biopsy
- Fibroblasts
- Humans
- Mice
- Oryzias*
- Rats
- Surgical Instruments
- Zebrafish
- PubMed
- 34551636 Full text @ Lab. Anim.
Citation
Beedgen, L., Hüllen, A., Gücüm, S., Thumberger, T., Wittbrodt, J., Thiel, C. (2021) A rapid and simple procedure for the isolation and cultivation of fibroblast-like cells from medaka and zebrafish embryos and fin clip biopsies. Laboratory animals. 56(3):270-278.
Abstract
In many human diseases, the molecular pathophysiological mechanisms are not understood, which makes the development and testing of new therapeutic approaches difficult. The generation and characterization of animal models such as mice, rats, fruit flies, worms or fish offers the possibility for in detail studies of a disease's development, its course and potential therapies in an organismal context, which considerably minimizes the risk of therapeutic side effects for patients. Nevertheless, due to the high numbers of experimental animals used in research worldwide, attempts to develop alternative test systems will help in reducing their count. In this regard, the cell culture system displays a suitable option due to its potential of delivering nearly unlimited material and the good opportunities for high-throughput studies such as drug testing. Here, we describe a quick and simple method to isolate and cultivate vital fibroblast-like cells from embryos and adults of two popular teleost model organisms, the Japanese rice fish medaka (Oryzias latipes) and the zebrafish (Danio rerio).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping