PUBLICATION
CRISPR/Cas9-Based Split Fluorescent Protein Tagging
- Authors
- Kesavan, G., Machate, A., Brand, M.
- ID
- ZDB-PUB-210909-10
- Date
- 2021
- Source
- Zebrafish 18(6): 369-373 (Journal)
- Registered Authors
- Brand, Michael, Kesavan, Gokul, Machate, Anja
- Keywords
- CRISPR/Cas9, genetically encoded fluorescent protein tags, knock-in, split fluorescent protein
- MeSH Terms
-
- Animals
- CRISPR-Associated Protein 9*/metabolism
- CRISPR-Cas Systems*
- Gene Editing
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 34495758 Full text @ Zebrafish
Citation
Kesavan, G., Machate, A., Brand, M. (2021) CRISPR/Cas9-Based Split Fluorescent Protein Tagging. Zebrafish. 18(6):369-373.
Abstract
Genetically encoded fluorescent tags such as green fluorescent protein fused to protein have revolutionized cell biology as they permit high-resolution protein imaging in live systems. Split fluorescent proteins, with a small fragment of 16 amino acids, can be inserted in the coding sequence to label proteins. We demonstrate successful integration of two bright and fast maturing split fluorescent proteins, mNeon green and sfCherry2, in zebrafish, and show that they are suitable for live imaging, including time-lapse series, and that they have a high signal-to-noise ratio. Furthermore, we show that CRISPR/Cas9 can be used to generate fluorescently tagged proteins in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping