PUBLICATION

Generation of a Transgenic Zebrafish Model for Pancreatic Beta Cell Regeneration

Authors
Pourghadamyari, H., Rezaei, M., Basiri, M., Tahamtani, Y., Asgari, B., Hassani, S.N., Meshkani, R., Golmohammadi, T., Baharvand, H.
ID
ZDB-PUB-210902-5
Date
2019
Source
Galen Medical Journal   8: e1056 (Journal)
Registered Authors
Pourghadamyari, Hossein, Rezaei, Mohammad, Tahamtani, Yaser
Keywords
Diabetes, Genetically Modified Animals, Pancreatic Beta Cells, Regeneration
MeSH Terms
none
PubMed
34466457 Full text @ Galen med. j.
Abstract
Background Diabetes is a major worldwide health problem. It is widely accepted that the beta cell mass decreases in type I diabetes (T1D). Accordingly, beta cell regeneration is a promising approach to increase the beta cell mass in T1D patients. However, the underlying mechanisms of beta cell regeneration have yet to be elucidated. One promising avenue is to create a relevant animal model to explore the underlying molecular and cellular mechanisms of beta cell regeneration. The zebrafish can be considered a model in beta cell regeneration studies because the pancreas structure and gene expression pattern are highly conserved between human and zebrafish.
Materials and methods In this study, the Tol2 transposase was exploited to generate a Tg(Ins:egfp-nfsB) zebrafish model that expressed a fusion protein composed of enhanced green fluorescent protein (EGFP) and nitroreductase (NTR) under control of the Ins promoter.
Results Metronidazole (MTZ) treatment of Tg(ins:egfp-nfsB) zebrafish larvae led to selective ablation of beta cells. Proof-of-concept evidence for beta cell regeneration in the transgenic larvae was observed two days after withdrawal of MTZ.
Conclusion This study suggests that the Tg(ins:egfp-nfsB) zebrafish can be used as a disease model to study beta cell regeneration and elucidate underlying mechanisms during the regeneration process.
Genes / Markers
Figures
Figure Gallery (4 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
zf3694Tg (TU)chemical treatment by environment: metronidazoleDay 4
zf3694Tg (TU)chemical treatment by environment: metronidazoleDay 4
zf3694Tg (TU)chemical treatment by environment: metronidazoleDay 4
1 - 3 of 3
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
zf3694TgTransgenic Insertion
    1 - 1 of 1
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    Human Disease / Model
    Human Disease Fish Conditions Evidence
    type 1 diabetes mellitusTAS
    1 - 1 of 1
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    Sequence Targeting Reagents
    No data available
    Fish
    Fish
    zf3694Tg (TU)
    1 - 1 of 1
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    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    EGFPEFGEGFP
    NTREFGNTR
    1 - 2 of 2
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    Mapping
    No data available
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    Citation
    Pourghadamyari, H., Rezaei, M., Basiri, M., Tahamtani, Y., Asgari, B., Hassani, S.N., Meshkani, R., Golmohammadi, T., Baharvand, H. (2019) Generation of a Transgenic Zebrafish Model for Pancreatic Beta Cell Regeneration. Galen Medical Journal. 8:e1056.