PUBLICATION

Apical contacts stemming from incomplete delamination guide progenitor cell allocation through a dragging mechanism

Authors
Pulgar, E., Schwayer, C., Guerrero, N., López, L., Márquez, S., Härtel, S., Soto, R., Heisenberg, C.P., Concha, M.
ID
ZDB-PUB-210828-43
Date
2021
Source
eLIFE   10: (Journal)
Registered Authors
Concha, Miguel, Heisenberg, Carl-Philipp
Keywords
developmental biology, zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Cell Adhesion
  • Cell Communication*
  • Cell Differentiation*
  • Cell Lineage
  • Cell Movement*
  • Embryo, Nonmammalian/physiology
  • Epithelial Cells/physiology*
  • Gene Expression Regulation, Developmental
  • Morphogenesis
  • Stem Cells/physiology*
  • Time Factors
  • Zebrafish/embryology
  • Zebrafish/genetics
PubMed
34448451 Full text @ Elife
Abstract
The developmental strategies used by progenitor cells to allow a safe journey from their induction place towards the site of terminal differentiation are still poorly understood. Here we uncovered a mechanism of progenitor cell allocation that stems from an incomplete process of epithelial delamination that allows progenitors to coordinate their movement with adjacent extra-embryonic tissues. Progenitors of the zebrafish laterality organ originate from the superficial epithelial enveloping layer by an apical constriction process of cell delamination. During this process, progenitors retain long-lasting apical contacts that enable the epithelial layer to pull a subset of progenitors on their way to the vegetal pole. The remaining delaminated cells follow the movement of apically attached progenitors by a protrusion-dependent cell-cell contact mechanism, avoiding sequestration by the adjacent endoderm, ensuring their collective fate and allocation at the site of differentiation. Thus, we reveal that incomplete delamination serves as a cellular platform for coordinated tissue movements during development.
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