PUBLICATION
Functional analysis of fis in Aeromonas veronii TH0426 reveals a key role in the regulation of virulence
- Authors
- Li, X., Song, H., Wang, J., Zhang, D., Shan, X., Yang, B., Kang, Y., Qian, A., Zhang, L., Sun, W.
- ID
- ZDB-PUB-210810-5
- Date
- 2021
- Source
- Microbial pathogenesis 159: 105123 (Journal)
- Registered Authors
- Keywords
- AAA+ family, Aeromonas veronii, Pathogenicity, Virulence, fis
- MeSH Terms
-
- Aeromonas*/genetics
- Aeromonas veronii/genetics
- Animals
- Aquaculture
- Carps*
- Gram-Negative Bacterial Infections*/veterinary
- Humans
- Virulence
- Zebrafish
- PubMed
- 34364977 Full text @ Microb. Pathog.
Citation
Li, X., Song, H., Wang, J., Zhang, D., Shan, X., Yang, B., Kang, Y., Qian, A., Zhang, L., Sun, W. (2021) Functional analysis of fis in Aeromonas veronii TH0426 reveals a key role in the regulation of virulence. Microbial pathogenesis. 159:105123.
Abstract
Aeromonas veronii is a comorbid pathogen that can infect humans, and animals including various aquatic organisms. In recent years, an increasing number of cases of A. veronii infection has been reported, indicating serious risks. This bacterium not only threatens public health and safety but also causes considerable economic loss in the aquaculture industry. Currently, some understanding of the pathogenic mechanism of A. veronii has been obtained. In this study, we first constructed the A. veronii TH0426 fis gene deletion strain Δfis and the complementation strain C-fis through homologous recombination technology. The results showed that the adhesion and invasion ability of the Δfis strain towards Epithelioma papulosum cyprini (EPC) cells and the cytotoxicity were 3.8-fold and 1.38-fold lower, respectively, than those of the wild-type strain. In the zebrafish infection model, the lethality of the deleted strain is 3-fold that of the wild strain. In addition, the bacterial load of the deletion strain Δfis in crucian carp was significantly lower than the wild-type strain, and the load decreased with time. In summary, deletion of the fis gene led to a decrease in the virulence of A. veronii. Our research results showed that the deletion of the fis gene significantly reduces the virulence and adhesion ability of A. veronii TH0426. Therefore, the fis gene plays a vital role in the pathogenesis of A. veronii TH0426. This preliminary study of the function of the fis gene in A. veronii will help researchers further understand the pathogenic mechanism of A. veronii.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping