PUBLICATION
A dandelion polysaccharide and its selenium nanoparticles: Structure features and evaluation of anti-tumor activity in zebrafish models
- Authors
- Zhang, S., Song, Z., Shi, L., Zhou, L., Zhang, J., Cui, J., Li, Y., Jin, D.Q., Ohizumi, Y., Xu, J., Guo, Y.
- ID
- ZDB-PUB-210810-4
- Date
- 2021
- Source
- Carbohydrate Polymers 270: 118365 (Journal)
- Registered Authors
- Cui, Jianlin
- Keywords
- Anti-tumor activity, Fructan, Selenium nanoparticles, Taraxacum mongolicum Hand.-Mazz, Zebrafish
- MeSH Terms
-
- A549 Cells
- Animals
- Antineoplastic Agents/pharmacology
- Apoptosis/drug effects
- Cell Proliferation/drug effects
- Fructans/chemistry
- Fructans/pharmacology
- HeLa Cells
- Hep G2 Cells
- Humans
- Hydrogen Bonding
- Nanoparticles/chemistry*
- Neoplasms/drug therapy*
- Neoplasms/metabolism
- Neoplasms/pathology
- Polysaccharides/chemistry
- Polysaccharides/pharmacology*
- Selenium/chemistry
- Selenium/pharmacology*
- Taraxacum/chemistry*
- Zebrafish
- PubMed
- 34364610 Full text @ Carbohydr. Polym.
Citation
Zhang, S., Song, Z., Shi, L., Zhou, L., Zhang, J., Cui, J., Li, Y., Jin, D.Q., Ohizumi, Y., Xu, J., Guo, Y. (2021) A dandelion polysaccharide and its selenium nanoparticles: Structure features and evaluation of anti-tumor activity in zebrafish models. Carbohydrate Polymers. 270:118365.
Abstract
In this study, an inulin fructan (TMP50-2) with moderate anti-tumor activity was obtained from dandelion. To further improve the anti-tumor activity of TMP50-2, a monodisperse and stable spherical nanoparticle (Tw-TMP-SeNP, 50 nm) was fabricated. Physico-chemical analysis revealed that TMP50-2 and Tween 80 were tightly wrapped on the surface of SeNPs by forming CO⋯Se bonds or through hydrogen bonding interaction (OH⋯Se). In vitro anti-tumor assay showed that Tw-TMP-SeNP treatment could significantly inhibit the proliferation of cancer cells (HepG2, A549, and HeLa) in a dose-dependent manner, while HepG2 cells were more susceptible to Tw-TMP-SeNP with an IC50 value of 46.8 μg/mL. The apoptosis induction of HepG2 cells by Tw-TMP-SeNP was evidenced by increasing the proportion of apoptotic cells ranging from 12.5% to 27.4%. Furthermore, in vivo zebrafish model confirmed the anti-tumor activity of Tw-TMP-SeNP by inhibiting the proliferation and migration of tumor cells as well as the angiogenesis of zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping