PUBLICATION
NIR-emitting styryl dyes with large Stokes' shifts for imaging application: From cellular plasma membrane, mitochondria to Zebrafish neuromast
- Authors
- Dahal, D., Ojha, K.R., Pokhrel, S., Paruchuri, S., Konopka, M., Liu, Q., Pang, Y.
- ID
- ZDB-PUB-210810-10
- Date
- 2021
- Source
- Dyes and pigments : an international journal 194: (Journal)
- Registered Authors
- Liu, Qin
- Keywords
- ESIPT, fluorescence imaging, mitochondria, near-infrared, neuromast, plasma membrane
- MeSH Terms
- none
- PubMed
- 34366501 Full text @ Dyes Pigm
Citation
Dahal, D., Ojha, K.R., Pokhrel, S., Paruchuri, S., Konopka, M., Liu, Q., Pang, Y. (2021) NIR-emitting styryl dyes with large Stokes' shifts for imaging application: From cellular plasma membrane, mitochondria to Zebrafish neuromast. Dyes and pigments : an international journal. 194:.
Abstract
Near-infrared (NIR) emitting probes with very large Stokes' shifts play a crucial role in bioimaging applications, as the optical signals in this region exhibit high signal to background ratio and allow deeper tissue penetration. Herein we illustrate NIR-emitting probe 2 with very large Stokes' shifts (Δλ ≈ 260 - 272 nm) by integrating the excited-state intramolecular proton transfer (ESIPT) unit 2-(2'-hydroxyphenyl)benzoxazole (HBO) into a pyridinium derived cyanine. The ESIPT not only enhances the Stokes' shifts but also improves the quantum efficiency of the probe 2 (фfl = 0.27 - 0.40 in DCM). The application of 2 in live cells imaging reveals that compound 2 stains mitochondria in eukaryotic cells, normal human lungs fibroblast (NHLF), Zebrafish's neuromast hair cells, and support cells, and inner plasma membrane in prokaryotic cells, Escherichia coli (E. coli).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping