PUBLICATION

Gjd2b-mediated gap junctions promote glutamatergic synapse formation and dendritic elaboration in Purkinje neurons

Authors
Sitaraman, S., Yadav, G., Agarwal, V., Jabeen, S., Verma, S., Jadhav, M., Thirumalai, V.
ID
ZDB-PUB-210805-2
Date
2021
Source
eLIFE   10: (Journal)
Registered Authors
Agarwal, Vandana, Jabeen, Shaista, Sitaraman, Sahana, Thirumalai, Vatsala, Yadav, Gnaneshwar
Keywords
neuroscience, zebrafish
MeSH Terms
  • Purkinje Cells/physiology*
  • Zebrafish/genetics
  • Zebrafish/physiology*
  • Connexins/genetics*
  • Connexins/metabolism
  • Neuronal Plasticity/physiology*
  • Gap Junctions/genetics*
  • Gap Junctions/metabolism
  • Eye Proteins/genetics*
  • Eye Proteins/metabolism
  • Synapses/physiology*
  • Animals
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
(all 14)
PubMed
34346310 Full text @ Elife
Abstract
Gap junctions between neurons serve as electrical synapses, in addition to conducting metabolites and signaling molecules. During development, early-appearing gap junctions are thought to prefigure chemical synapses, which appear much later. We present evidence for this idea at a central, glutamatergic synapse and provide some mechanistic insights. Loss or reduction in the levels of the gap junction protein Gjd2b decreased the frequency of glutamatergic miniature excitatory postsynaptic currents (mEPSCs) in cerebellar Purkinje neurons (PNs) in larval zebrafish. Ultrastructural analysis in the molecular layer showed decreased synapse density. Further, mEPSCs had faster kinetics and larger amplitudes in mutant PNs, consistent with their stunted dendritic arbors. Time-lapse microscopy in wild type and mutant PNs reveals that Gjd2b puncta promote the elongation of branches and that CaMKII may be a critical mediator of this process. These results demonstrate that Gjd2b-mediated gap junctions regulate glutamatergic synapse formation and dendritic elaboration in PNs.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ncb200
    Indel
    ncb204
      Indel
      ncb207
        Indel
        ncb214
          Indel
          ncb215
            Insertion
            rk22TgTransgenic Insertion
              1 - 6 of 6
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              Human Disease / Model
              No data available
              Sequence Targeting Reagents
              Target Reagent Reagent Type
              gjd2bMO1-gjd2bMRPHLNO
              gjd2bTALEN1-gjd2bTALEN
              1 - 2 of 2
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              Fish
              Antibodies
              Name Type Antigen Genes Isotypes Host Organism
              Ab1-cx35monoclonalIgG1Mouse
              Ab1-pvalbmonoclonal
                IgG1Mouse
                1 - 2 of 2
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                Orthology
                No data available
                Engineered Foreign Genes
                Marker Marker Type Name
                VenusEFGVenus
                1 - 1 of 1
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                Mapping
                No data available