PUBLICATION
Temporal single-cell transcriptomes of zebrafish spinal cord pMN progenitors reveal distinct neuronal and glial progenitor populations
- Authors
- Scott, K., O'Rourke, R., Winkler, C.C., Kearns, C.A., Appel, B.
- ID
- ZDB-PUB-210728-25
- Date
- 2021
- Source
- Developmental Biology 479: 37-50 (Journal)
- Registered Authors
- Appel, Bruce, Kearns, Christina
- Keywords
- Motor neurons, Neural development, Neural progenitors, Oligodendrocyte, Zebrafish
- Datasets
- GEO:GSE173350
- MeSH Terms
-
- Animals
- Basic Helix-Loop-Helix Transcription Factors/genetics
- Cell Differentiation/physiology*
- Cell Lineage
- Gene Expression Profiling/methods
- Gene Expression Regulation, Developmental/genetics
- Hedgehog Proteins/metabolism
- Motor Neurons/cytology
- Nerve Tissue Proteins/metabolism
- Neural Stem Cells/cytology*
- Neurogenesis/physiology
- Oligodendroglia/cytology
- Single-Cell Analysis/methods
- Spatio-Temporal Analysis
- Spinal Cord/embryology*
- Transcription Factors/metabolism
- Transcriptome/genetics
- Zebrafish/embryology
- Zebrafish Proteins/genetics
- PubMed
- 34303700 Full text @ Dev. Biol.
Citation
Scott, K., O'Rourke, R., Winkler, C.C., Kearns, C.A., Appel, B. (2021) Temporal single-cell transcriptomes of zebrafish spinal cord pMN progenitors reveal distinct neuronal and glial progenitor populations. Developmental Biology. 479:37-50.
Abstract
Ventral spinal cord progenitor cells, which express the basic helix loop helix transcription factor Olig2, sequentially produce motor neurons and oligodendrocyte precursor cells (OPCs). Following specification some OPCs differentiate as myelinating oligodendrocytes while others persist as OPCs. Though a considerable amount of work has described the molecular profiles that define motor neurons, OPCs, and oligodendrocytes, less is known about the progenitors that produce them. To identify the developmental origins and transcriptional profiles of motor neurons and OPCs, we performed single-cell RNA sequencing on isolated pMN cells from embryonic zebrafish trunk tissue at stages that encompassed motor neurogenesis, OPC specification, and initiation of oligodendrocyte differentiation. Downstream analyses revealed two distinct pMN progenitor populations: one that appears to produce neurons and one that appears to produce OPCs. This latter population, called Pre-OPCs, is marked by expression of GS Homeobox 2 (gsx2), a gene that encodes a homeobox transcription factor. Using fluorescent in situ hybridizations, we identified gsx2-expressing Pre-OPCs in the spinal cord prior to expression of canonical OPC marker genes. Our data therefore reveal heterogeneous gene expression profiles among pMN progenitors, supporting prior fate mapping evidence.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping