PUBLICATION
Enantioselective Toxicity Effects of 2,2',3,5',6-Pentachloro Biphenyl (PCB-95) on Developing Brains in Zebrafish Larvae
- Authors
- Ranasinghe, P., Thorn, R.J., Creton, R., Lee, C.M.
- ID
- ZDB-PUB-210708-3
- Date
- 2021
- Source
- Bulletin of environmental contamination and toxicology 107(2): 351-360 (Journal)
- Registered Authors
- Creton, Robbert
- Keywords
- Brain cell lesions, Brain size, Neurodevelopment, PCB-95, Reactive oxygen Species
- MeSH Terms
-
- Animals
- Biphenyl Compounds
- Brain
- Larva
- Polychlorinated Biphenyls*/toxicity
- Stereoisomerism
- Zebrafish*
- PubMed
- 34230987 Full text @ Bull. Environ. Contam. Toxicol.
Citation
Ranasinghe, P., Thorn, R.J., Creton, R., Lee, C.M. (2021) Enantioselective Toxicity Effects of 2,2',3,5',6-Pentachloro Biphenyl (PCB-95) on Developing Brains in Zebrafish Larvae. Bulletin of environmental contamination and toxicology. 107(2):351-360.
Abstract
2,2',3,5',6-Pentachlorobiphenyl (PCB-95) is an environmentally relevant, chiral PCB congener that has been shown to act as a developmental neurotoxicant (DNT), targeting the developing brain. However, understanding enantioselective toxic effects for PCB-95 is in its infancy. To investigate these toxic effects, zebrafish embryos were exposed to racemates and enantiomers of PCB-95. Brain areas and pathology were studied. Results indicated dose dependent reduction of brain sizes with increased brain cell death in racemic and Ra (-)-PCB-95 treated groups. To provide a mechanistic basis for the observed neurotoxicity, gene expressions of antioxidant proteins such as Cu/Zn-SOD, Mn-SOD, and GPx were analysed. Antioxidant genes were up regulated with the PCB-95 exposure and racemic PCB-95 showed higher toxicity. These results suggest that the exposure to PCB-95 contributed to developmental neurotoxicity in early developing zebrafish larvae and may confer risks associated with enantioselective enrichment of PCB-95 in the environment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping