PUBLICATION
Identification of the causative gene of a transparent phenotype of juvenile red sea bream Pagrus major
- Authors
- Sawayama, E., Handa, Y., Nakano, K., Noguchi, D., Takagi, M., Akiba, Y., Sanada, S., Yoshizaki, G., Usui, H., Kawamoto, K., Suzuki, M., Asahina, K.
- ID
- ZDB-PUB-210629-27
- Date
- 2021
- Source
- Heredity 127(2): 167-175 (Journal)
- Registered Authors
- Yoshizaki, Goro
- Keywords
- none
- MeSH Terms
-
- Animals
- Genetic Linkage
- Humans
- Phenotype
- Sea Bream*/genetics
- Thyroid Hormones
- Zebrafish
- PubMed
- 34175895 Full text @ Heredity
Citation
Sawayama, E., Handa, Y., Nakano, K., Noguchi, D., Takagi, M., Akiba, Y., Sanada, S., Yoshizaki, G., Usui, H., Kawamoto, K., Suzuki, M., Asahina, K. (2021) Identification of the causative gene of a transparent phenotype of juvenile red sea bream Pagrus major. Heredity. 127(2):167-175.
Abstract
Deformities in cultured fish species may be genetic, and identifying causative genes is essential to expand production and maintain farmed animal welfare. We previously reported a genetic deformity in juvenile red sea bream, designated a transparent phenotype. To identify its causative gene, we conducted genome-wide linkage analysis and identified two single nucleotide polymorphisms (SNP) located on LG23 directly linked to the transparent phenotype. The scaffold on which the two SNPs were located contained two candidate genes, duox and duoxa, which are related to thyroid hormone synthesis. Four missense mutations were found in duox and one in duoxa, with that in duoxa showing perfect association with the transparent phenotype. The mutation of duoxa was suggested to affect the transmembrane structure and thyroid-related traits, including an enlarged thyroid gland and immature erythrocytes, and lower thyroxine (T4) concentrations were observed in the transparent phenotype. The transparent phenotype was rescued by T4 immersion. Loss-of-function of duoxa by CRISPR-Cas9 induced the transparent phenotype in zebrafish. Evidence suggests that the transparent phenotype of juvenile red sea bream is caused by the missense mutation of duoxa and that this mutation disrupts thyroid hormone synthesis. The newly identified missense mutation will contribute to effective selective breeding of red sea bream to purge the causative gene of the undesirable phenotype and improve seed production of red sea bream as well as provide basic information of the mechanisms of thyroid hormones and its related diseases in fish and humans.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping