PUBLICATION

Occurrence of foamy macrophages during the innate response of zebrafish to trypanosome infections

Authors
Jacobs, S.H., Dóró, E., Hammond, F.R., Nguyen-Chi, M.E., Lutfalla, G., Wiegertjes, G.F., Forlenza, M.
ID
ZDB-PUB-210612-2
Date
2021
Source
eLIFE   10: (Journal)
Registered Authors
Lutfalla, Georges
Keywords
infectious disease, microbiology, zebrafish
MeSH Terms
  • Humans
  • Inflammation/immunology
  • Phagocytosis
  • Macrophages/immunology*
  • Macrophages/metabolism
  • Neutrophils/immunology*
  • Neutrophils/metabolism
  • Cell Proliferation
  • Trypanosoma/immunology*
  • Disease Models, Animal
  • Immunity, Innate
  • Zebrafish/immunology
  • Animals
  • Trypanosomiasis/immunology*
  • Larva/immunology
(all 15)
PubMed
34114560 Full text @ Elife
Abstract
A tightly regulated innate immune response to trypanosome infections is critical to strike a balance between parasite control and inflammation-associated pathology. In this study, we make use of the recently established Trypanosoma carassii infection model in larval zebrafish to study the early response of macrophages and neutrophils to trypanosome infections in vivo. We consistently identified high- and low-infected individuals and were able to simultaneously characterize their differential innate response. Not only did macrophage and neutrophil number and distribution differ between the two groups, but also macrophage morphology and activation state. Exclusive to high-infected zebrafish, was the occurrence of foamy macrophages characterized by a strong pro-inflammatory profile and potentially associated with an exacerbated immune response as well as susceptibility to the infection. To our knowledge this is the first report of the occurrence of foamy macrophages during an extracellular trypanosome infection.
Genes / Markers
Figures
Figure Gallery (13 images) / 2
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
EGFPgl22Tg/gl22Tg; ump2Tg/ump2Tgchemical treatment by environment: poly(vinylpyrrolidone)1k-cell to Days 14-20IFL
EGFPgl22Tg/gl22Tg; ump2Tg/ump2Tgbiological treatment: Trypanosoma carassii1k-cell to Days 14-20IFL
EGFPump2Tg/ump2Tg; ump3Tg/ump3Tgbiological treatment: Trypanosoma carassiiDay 5IFL
EGFPump2Tg/ump2Tg; ump3Tg/ump3Tgbiological treatment: Trypanosoma carassiiDays 7-13IFL
EGFPump2Tg/ump2Tg; ump5Tg/ump5Tgbiological treatment: Trypanosoma carassiiDay 5IFL
EGFPump2Tg/ump2Tg; ump5Tg/ump5Tgbiological treatment: Trypanosoma carassiiDays 7-13IFL
GFPi114Tg/i114Tg; s896Tg/s896Tgbiological treatment: Trypanosoma carassii1k-cell to Days 14-20IFL
mCherrygl22Tg/gl22Tg; ump2Tg/ump2Tgbiological treatment: Trypanosoma carassii1k-cell to Days 14-20IFL
mCherrygl22Tg/gl22Tg; ump2Tg/ump2Tgchemical treatment by environment: poly(vinylpyrrolidone)1k-cell to Days 14-20IFL
mCherryump2Tg/ump2Tg; ump3Tg/ump3Tgbiological treatment: Trypanosoma carassiiDays 7-13IFL
1 - 10 of 16
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Phenotype
Fish Conditions Stage Phenotype Figure
gl22Tg/gl22Tg; ump2Tg/ump2Tgchemical treatment by environment: poly(vinylpyrrolidone)Protruding-mouth to Days 14-20
gl22Tg/gl22Tg; ump2Tg/ump2Tgchemical treatment by environment: poly(vinylpyrrolidone)Protruding-mouth to Days 14-20
gl22Tg/gl22Tg; ump2Tg/ump2Tgchemical treatment by environment: poly(vinylpyrrolidone)Day 5 to Days 14-20
gl22Tg/gl22Tg; ump2Tg/ump2Tgbiological treatment: Trypanosoma carassii1k-cell to Days 14-20
gl22Tg/gl22Tg; ump2Tg/ump2Tgbiological treatment: Trypanosoma carassiiProtruding-mouth to Days 14-20
gl22Tg/gl22Tg; ump2Tg/ump2Tgbiological treatment: Trypanosoma carassiiProtruding-mouth to Days 14-20
gl22Tg/gl22Tg; ump2Tg/ump2Tgbiological treatment: Trypanosoma carassiiProtruding-mouth to Days 14-20
gl22Tg/gl22Tg; ump2Tg/ump2Tgbiological treatment: Trypanosoma carassiiProtruding-mouth to Days 14-20
gl22Tg/gl22Tg; ump2Tg/ump2Tgbiological treatment: Trypanosoma carassiiProtruding-mouth to Days 14-20
gl22Tg/gl22Tg; ump2Tg/ump2Tgbiological treatment: Trypanosoma carassiiProtruding-mouth to Days 14-20
1 - 10 of 46
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
a9
    		Complex
    gl22TgTransgenic Insertion
      i114TgTransgenic Insertion
        s896TgTransgenic Insertion
          ump2TgTransgenic Insertion
            ump3TgTransgenic Insertion
              ump5TgTransgenic Insertion
                w2
                  		Point Mutation
                  y1TgTransgenic Insertion
                    1 - 9 of 9
                    Show
                    Human Disease / Model
                    No data available
                    Sequence Targeting Reagents
                    No data available
                    Fish
                    Fish
                    gl22Tg/gl22Tg; ump2Tg/ump2Tg
                    i114Tg/i114Tg; s896Tg/s896Tg
                    i114Tg/i114Tg; ump2Tg/ump2Tg
                    ump2Tg/ump2Tg; ump3Tg/ump3Tg
                    ump2Tg/ump2Tg; ump5Tg/ump5Tg
                    ump2Tg/ump2Tg; y1Tg/y1Tg
                    1 - 6 of 6
                    Show
                    Antibodies
                    No data available
                    Orthology
                    No data available
                    Engineered Foreign Genes
                    Marker Marker Type Name
                    EGFPEFGEGFP
                    GFPEFGGFP
                    mCherryEFGmCherry
                    1 - 3 of 3
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                    Mapping
                    No data available
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                    Citation
                    Jacobs, S.H., Dóró, E., Hammond, F.R., Nguyen-Chi, M.E., Lutfalla, G., Wiegertjes, G.F., Forlenza, M. (2021) Occurrence of foamy macrophages during the innate response of zebrafish to trypanosome infections. eLIFE. 10:.