PUBLICATION
Exogenous Expression of pou5f3 Facilitates the Development of Somatic Cell Nuclear Transfer Embryos in Zebrafish at the Early Stage
- Authors
- Wang, X., Zhao, J., Zhu, Z.
- ID
- ZDB-PUB-210609-10
- Date
- 2021
- Source
- Cellular reprogramming 23(3): 191-197 (Journal)
- Registered Authors
- Zhao, Jue
- Keywords
- embryo development, pou5f3, reprogramming, somatic cell nuclear transfer, zebrafish
- MeSH Terms
-
- Animals
- Blastocyst/cytology*
- Blastocyst/metabolism
- Cellular Reprogramming*
- Embryo, Nonmammalian/cytology*
- Embryo, Nonmammalian/metabolism
- Embryonic Development*
- Nuclear Transfer Techniques*
- Octamer Transcription Factor-3/genetics
- Octamer Transcription Factor-3/metabolism*
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 34101505 Full text @ Cell Reprogram
Citation
Wang, X., Zhao, J., Zhu, Z. (2021) Exogenous Expression of pou5f3 Facilitates the Development of Somatic Cell Nuclear Transfer Embryos in Zebrafish at the Early Stage. Cellular reprogramming. 23(3):191-197.
Abstract
Enucleated oocytes can reprogram differentiated nuclei to totipotency after somatic cell nuclear transfer (SCNT), which is valuable in understanding nuclear reprogramming and generating genetically modified animals. To date, reprogramming efficiency is low and the development of SCNT embryos is not going as well as anticipated. To further disclose the reprogramming mechanisms during SCNT zebrafish embryo development, we examined the expression patterns of transcription regulation factors and regulated them by mRNA and morpholino microinjection. In this study, we show that stem cell-related transcription factors are downregulated in zebrafish SCNT embryos at the blastula stage. Exogenous expression of pou5f3 at the single-cell stage improves SCNT embryo development from the blastula to the gastrula stage. We also found that exogenous expression of klf4 or sox2 decreases SCNT embryo development from the blastula to the gastrula stage, while expression of nanog is necessary for the development of SCNT embryos. Our results conclude that zebrafish pou5f3 facilitates the development of SCNT embryos from the blastula to gastrula stage.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping