PUBLICATION
Amelioration of cognitive deficit in zebrafish by an undescribed anthraquinone from Juglans regia L.: An in-silico, in-vitro and in-vivo approach
- Authors
- Devidas, S.B., Rahmatkar, S.N., Singh, R., Sendri, N., Purohit, R., Singh, D., Bhandari, P.
- ID
- ZDB-PUB-210608-4
- Date
- 2021
- Source
- European Journal of Pharmacology 906: 174234 (Journal)
- Registered Authors
- Keywords
- Acetylcholinesterase, Anthraquinone, Juglans regia L, Neurotoxicity, T-maze, Zebrafish
- MeSH Terms
-
- Acetylcholinesterase/metabolism
- Acrylamide/administration & dosage
- Acrylamide/toxicity
- Animals
- Anthraquinones/isolation & purification
- Anthraquinones/pharmacology*
- Anthraquinones/therapeutic use
- Cholinesterase Inhibitors/isolation & purification
- Cholinesterase Inhibitors/pharmacology*
- Cholinesterase Inhibitors/therapeutic use
- Cognitive Dysfunction/chemically induced
- Cognitive Dysfunction/prevention & control*
- Disease Models, Animal
- Humans
- Juglans/chemistry
- Learning/drug effects
- Memory/drug effects
- Molecular Docking Simulation
- Neuroprotective Agents/isolation & purification
- Neuroprotective Agents/pharmacology*
- Neuroprotective Agents/therapeutic use
- Zebrafish
- PubMed
- 34090895 Full text @ Eur. J. Pharmacol.
Citation
Devidas, S.B., Rahmatkar, S.N., Singh, R., Sendri, N., Purohit, R., Singh, D., Bhandari, P. (2021) Amelioration of cognitive deficit in zebrafish by an undescribed anthraquinone from Juglans regia L.: An in-silico, in-vitro and in-vivo approach. European Journal of Pharmacology. 906:174234.
Abstract
An undescribed anthraquinone assigned as 1-Hydroxy-5,5-dimethyl-5,6,7,8-tetrahydro-9,10-anthraquinone (compound 1) was isolated from ethylacetate extract of Juglans regia. The structure of the compound was established on the basis of 1D, 2D NMR (HSQC, HMBC, COSY), ESI-QTOF-MS/MS spectroscopy. The molecular docking studies of compound 1 indicated similar molecular interactions as that of co-crystalized inhibitor. Compound 1 showed hydrogen bonds with residues PHE295, GLY121, π-σ interactions with TYR 341, π-π interactions with HIS 447 residues, and π-alkyl with TRP86 and TYR 337. On the basis of in-silico interaction studies of compound 1 with proteins, it was tested using acetylcholinesterase inhibition assay, acrylamide-induced neurotoxicity test of zebrafish larva, and scopolamine-induced cognitive deficit model of adult zebrafish. The compound 1 showed potent acetylcholinesterase inhibition activity, prevented acrylamide-induced neurotoxicity and improved learning and memory functions in T-maze test. The results established compound 1 to be a potential neuroprotective natural product for amelioration of cognitive impairment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping