PUBLICATION

Developmental exposure to domoic acid disrupts startle response behavior and circuitry in zebrafish

Authors
Panlilio, J.M., Jones, I.T., Salanga, M.C., Aluru, N., Hahn, M.E.
ID
ZDB-PUB-210608-26
Date
2021
Source
Toxicological sciences : an official journal of the Society of Toxicology   182(2): 310-326 (Journal)
Registered Authors
Panlilio, Jennifer Martinez, Salanga, Matthew
Keywords
Domoic acid, developmental toxicity, escape response, harmful algal bloom toxins, harmful algal blooms, startle circuit, startle response
MeSH Terms
  • Adult
  • Animals
  • Humans
  • Kainic Acid/analogs & derivatives
  • Kainic Acid/toxicity
  • Neurons
  • Reflex, Startle*
  • Zebrafish*
PubMed
34097058 Full text @ Toxicol. Sci.
Abstract
Harmful algal blooms produce potent neurotoxins that accumulate in seafood and are hazardous to human health. Developmental exposure to the harmful algal bloom toxin, domoic acid (DomA), has behavioral consequences well into adulthood, but the cellular and molecular mechanisms of DomA developmental neurotoxicity are largely unknown. To assess these, we exposed zebrafish embryos to DomA during the previously identified window of susceptibility and used the well-known startle response circuit as a tool to identify specific neuronal components that are targeted by exposure to DomA. Exposure to DomA reduced startle responsiveness to both auditory/vibrational and electrical stimuli, and even at the highest stimulus intensities tested, led to a dramatic reduction of one type of startle (short latency c-starts). Furthermore, DomA-exposed larvae had altered kinematics for both types of startle responses tested, exhibiting shallower bend angles and slower maximal angular velocities. Using vital dye staining, immunolabelling, and live imaging of transgenic lines, we determined that while the sensory inputs were intact, the reticulospinal neurons required for short latency c-starts were absent in most DomA-exposed larvae. Furthermore, axon tracing revealed that DomA-treated larvae also showed significantly reduced primary motor neuron axon collaterals. Overall, these results show that developmental exposure to DomA targets large reticulospinal neurons and motor neuron axon collaterals, resulting in measurable deficits in startle behavior. They further provide a framework for using the startle response circuit to identify specific neural populations disrupted by toxins or toxicants and to link these disruptions to functional consequences for neural circuit function and behavior.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping