PUBLICATION
Iron ion sensing and in vitro and in vivo imaging based on bright blue-fluorescent carbon dots
- Authors
- Chang, D., Zhao, Z., Niu, W., Shi, L., Yang, Y.
- ID
- ZDB-PUB-210601-7
- Date
- 2021
- Source
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 260: 119964 (Journal)
- Registered Authors
- Zhao, Zhonghua
- Keywords
- Blue fluorescence, Carbon dots, Imaging, Iron ion, Sensing
- MeSH Terms
-
- Animals
- Carbon*
- Fluorescent Dyes
- Iron
- Mice
- Mice, Nude
- Quantum Dots*/toxicity
- Spectrometry, Fluorescence
- Zebrafish
- PubMed
- 34052767 Full text @ Spectrochim Acta A Mol Biomol Spectrosc.
Citation
Chang, D., Zhao, Z., Niu, W., Shi, L., Yang, Y. (2021) Iron ion sensing and in vitro and in vivo imaging based on bright blue-fluorescent carbon dots. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 260:119964.
Abstract
Herein, we propose an eco-friendly synthesis of carbon dots (CDs) and ingeniously design a rapid and label-free "turn-off" sensing platform for ultrasensitive recognition of Fe3+ in vitro and in vivo. CDs with extraordinary advantages involving exceptional stability, ultra-low toxicity as well as admirable biocompatibility were simply prepared via one-step hydrothermal strategy of Caulis polygoni multiflora. Result indicated that as-acquired CDs not only exhibit excitation dependency, but also have a high quantum yield of (QY) up to 42%. Miraculously, the fluorescence of CDs can be extinguished sharply by Fe3+ because of static quenching effect with linear range of 0-400 µM, yielding a detection limit of 0.025 μM. Benefiting from these characteristics, CDs have been extended for multicolourful imaging and tracking Fe3+ fluctuations in living cells. Bioimaging of zebrafish larvae exposed to CDs confirmed that it is smoothly circulated to other tissues and organs owing to their small size. Eventually, as-prepared CDs have been implemented for the real-time detection of Fe3+ in nude mice.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping