PUBLICATION

Myeloid-derived growth factor regulates neutrophil motility in interstitial tissue damage

Authors
Houseright, R.A., Miskolci, V., Mulvaney, O., Bortnov, V., Mosher, D.F., Rindy, J., Bennin, D.A., Huttenlocher, A.
ID
ZDB-PUB-210529-11
Date
2021
Source
The Journal of cell biology   220(8): (Journal)
Registered Authors
Huttenlocher, Anna
Keywords
none
MeSH Terms
  • Animal Fins/injuries
  • Animal Fins/metabolism*
  • Animal Fins/microbiology
  • Animal Fins/pathology
  • Animals
  • Animals, Genetically Modified
  • Cell Movement*
  • Disease Models, Animal
  • Hypoxia-Inducible Factor 1, alpha Subunit/genetics
  • Hypoxia-Inducible Factor 1, alpha Subunit/metabolism
  • Inflammation/genetics
  • Inflammation/metabolism*
  • Inflammation/microbiology
  • Macrophages/metabolism
  • Macrophages/microbiology
  • Microscopy, Fluorescence
  • Neutrophil Infiltration*
  • Neutrophils/metabolism*
  • Neutrophils/microbiology
  • Paracrine Communication
  • Pseudomonas aeruginosa/pathogenicity
  • Signal Transduction
  • Time Factors
  • Wound Healing*
  • Wound Infection/genetics
  • Wound Infection/metabolism*
  • Wound Infection/microbiology
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
34047769 Full text @ J. Cell Biol.
Abstract
Neutrophil recruitment to tissue damage is essential for host defense but can also impede tissue repair. The cues that differentially regulate neutrophil responses to tissue damage and infection remain unclear. Here, we report that the paracrine factor myeloid-derived growth factor (MYDGF) is induced by tissue damage and regulates neutrophil motility to damaged, but not infected, tissues in zebrafish larvae. Depletion of MYDGF impairs wound healing, and this phenotype is rescued by depleting neutrophils. Live imaging and photoconversion reveal impaired neutrophil reverse migration and inflammation resolution in mydgf mutants. We found that persistent neutrophil inflammation in tissues of mydgf mutants was dependent on the HIF-1α pathway. Taken together, our data suggest that MYDGF is a damage signal that regulates neutrophil interstitial motility and inflammation through a HIF-1α pathway in response to tissue damage.
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