PUBLICATION
Zebrafish Tumor Graft Transplantation to Grow Tumors In Vivo That Engraft Poorly as Single Cell Suspensions
- Authors
- Lipsitt, A., Hensch, N.R., Moreno-Campos, R., Baxi, K., Chen, J., Challa, A.K., Chen, E.Y., Ignatius, M.S.
- ID
- ZDB-PUB-210526-4
- Date
- 2021
- Source
- Zebrafish 18(4): 293-296 (Journal)
- Registered Authors
- Challa, Anil Kumar
- Keywords
- angiosarcoma, tp53, tumor graft, zebrafish, zebrafish cancer model
- MeSH Terms
-
- Animals
- Disease Models, Animal
- Hemangiosarcoma*/pathology
- Neoplasm Transplantation*
- Suspensions
- Tumor Microenvironment
- Zebrafish*
- PubMed
- 34030492 Full text @ Zebrafish
Citation
Lipsitt, A., Hensch, N.R., Moreno-Campos, R., Baxi, K., Chen, J., Challa, A.K., Chen, E.Y., Ignatius, M.S. (2021) Zebrafish Tumor Graft Transplantation to Grow Tumors In Vivo That Engraft Poorly as Single Cell Suspensions. Zebrafish. 18(4):293-296.
Abstract
Angiosarcoma is a clinically aggressive tumor with a high rate of mortality. It can arise in vascular or lymphatic tissues, involve any part of the body, and aggressively spread locally or metastasize. Angiosarcomas spontaneously develop in the tp53 deleted (tp53del/del) zebrafish mutant. However, established protocols for tumor dissection and transplantation of single cell suspensions of angiosarcoma tumors result in inferior implantation rates. To resolve these complications, we developed a new tumor grafting technique for engraftment of angiosarcoma and similar tumors in zebrafish, which maintains the tumor microenvironment and has superior rates of engraftment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping