PUBLICATION
Alternate Immersion in Glucose to Produce Prolonged Hyperglycemia in Zebrafish
- Authors
- McCarthy, E., Rowe, C.J., Crowley-Perry, M., Connaughton, V.P.
- ID
- ZDB-PUB-210525-13
- Date
- 2021
- Source
- Journal of visualized experiments : JoVE (171): (Journal)
- Registered Authors
- Connaughton, Victoria P.
- Keywords
- none
- MeSH Terms
-
- Animals
- Blood Glucose
- Diabetes Mellitus, Type 2*
- Disease Models, Animal
- Glucose
- Hyperglycemia*/chemically induced
- Immersion
- Zebrafish
- PubMed
- 34028434 Full text @ J. Vis. Exp.
Citation
McCarthy, E., Rowe, C.J., Crowley-Perry, M., Connaughton, V.P. (2021) Alternate Immersion in Glucose to Produce Prolonged Hyperglycemia in Zebrafish. Journal of visualized experiments : JoVE. (171):.
Abstract
Zebrafish (Danio rerio) are an excellent model to investigate the effects of chronic hyperglycemia, a hallmark of Type II Diabetes Mellitus (T2DM). This alternate immersion protocol is a noninvasive, step-wise method of inducing hyperglycemia for up to eight weeks. Adult zebrafish are alternately exposed to sugar (glucose) and water for 24 hours each. The zebrafish begin treatment in a 1% glucose solution for 2 weeks, then a 2% solution for 2 weeks, and finally a 3% solution for the remaining 4 weeks. Compared to water-treated (stress) and mannitol-treated (osmotic) controls, glucose-treated zebrafish have significantly higher blood sugar levels. The glucose-treated zebrafish show blood sugar levels of 3-times that of controls, suggesting that after both four and eight weeks hyperglycemia can be achieved. Sustained hyperglycemia was associated with increased Glial Fibrillary Acidic Protein (GFAP) and increased nuclear factor Kappa B (NF-kB) levels in retina and decreased physiological responses, as well as cognitive deficits suggesting this protocol can be used to model disease complications.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping