PUBLICATION

Zebrafish twist2/dermo1 regulates scale shape and scale organization during skin development and regeneration

Authors

Jacob, T., Chakravarty, A., Panchal, A., Patil, M., Ghodadra, G., Sudhakaran, J., Nuesslein-Volhard, C.

ID

ZDB-PUB-210519-8

Date

2021

Source

Cells & development 166: 203684 (Journal)

Registered Authors

Jacob, Tressa Panikulangara

Keywords

Metamorphosis, Regeneration, Scales, Wnt signaling, twist2/dermo1

MeSH Terms

Wnt Signaling Pathway
Animals
Regeneration/physiology*
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism*
Animal Scales/anatomy & histology*
Twist-Related Protein 2/genetics
Twist-Related Protein 2/metabolism*
Genotype
Skin/embryology*
Zebrafish/embryology*
Zebrafish/genetics*
Base Sequence
Phenotype
Mutation/genetics
Gene Expression Regulation, Developmental

(all 16)

PubMed

33994357 Full text @ Cells Dev

Abstract

Scales are skin appendages in fishes that evolutionarily predate feathers in birds and hair in mammals. Zebrafish scales are dermal in origin and develop during metamorphosis. Understanding regulation of scale development in zebrafish offers an exciting possibility of unraveling how the mechanisms of skin appendage formation evolved in lower vertebrates and whether these mechanisms remained conserved in birds and mammals. Here we have investigated the expression and function of twist 2/dermo1 gene - known for its function in feather and hair formation - in scale development and regeneration. We show that of the four zebrafish twist paralogues, twist2/dermo1 and twist3 are expressed in the scale forming cells during scale development. Their expression is also upregulated during scale regeneration. Our knockout analysis reveals that twist2/dermo1 gene functions in the maintenance of the scale shape and organization during development as well as regeneration. We further show that the expression of twist2/dermo1 and twist3 is regulated by Wnt signaling. Our results demonstrate that the function of twist2/dermo1 in skin appendage formation, presumably under regulation of Wnt signaling, originated during evolution of basal vertebrates.

Genes / Markers

Figures

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Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
TU	chemical treatment by environment: IWR-1-endo	Days 21-29	scale primordium twist2 expression absent, abnormal	Fig. 5
TU	chemical treatment by environment: IWR-1-endo	Days 21-29	scale primordium twist3 expression spatial pattern, abnormal	Fig. 5
TU	chemical treatment by environment: IWR-1-endo	Days 30-44	scale twist2 expression absent, abnormal	Fig. 5
TU	chemical treatment by environment: IWR-1-endo	Days 30-44	scale twist3 expression spatial pattern, abnormal	Fig. 5
TU	abrasion	Adult	scale twist2 expression increased amount, abnormal	Fig. 2
TU	abrasion	Adult	scale twist3 expression increased amount, abnormal	Fig. 2
twist2^{zf3440/zf3440}	standard conditions	Days 30-44	scale 2-D shape, abnormal	Fig. 3
twist2^{zf3440/zf3440}	standard conditions	Days 30-44	scale disorganized, abnormal	Fig. 3
twist2^{zf3440/zf3440}	standard conditions	Days 30-44	scale skin epidermis development decreased process quality, abnormal	Fig. 3
twist2^{zf3440/zf3440}	standard conditions	Adult	scale tissue regeneration decreased process quality, abnormal	Fig. 4

1 - 10 of 11

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
zf106Tg	Tg(-8.0cldnb:LY-EGFP)	Transgenic Insertion
zf3440		Point Mutation	twist2

Human Disease / Model

Sequence Targeting Reagents

Fish

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP

Mapping

Jacob et al., 2021 ZDB-PUB-210519-8 PMID:33994357

Zebrafish twist2/dermo1 regulates scale shape and scale organization during skin development and regeneration

Jacob et al., 2021

ZDB-PUB-210519-8
PMID:33994357