PUBLICATION
LncRNA FAM230B promotes the metastasis of papillary thyroid cancer by sponging the miR-378a-3p/WNT5A axis
- Authors
- Zhou, Q., Feng, J., Yin, S., Ma, S., Wang, J., Yi, H.
- ID
- ZDB-PUB-210518-1
- Date
- 2021
- Source
- Biochemical and Biophysical Research Communications 546: 83-89 (Journal)
- Registered Authors
- Keywords
- Metastasis, Papillary thyroid cancer, WNT5A, lncRNA FAM230B, miR-378a-3p
- MeSH Terms
-
- Animals
- Cell Line, Tumor
- Cell Movement/genetics
- Gene Expression Regulation, Neoplastic
- Humans
- MicroRNAs/genetics*
- Neoplasm Invasiveness/genetics
- Neoplasm Metastasis/genetics*
- RNA, Long Noncoding/genetics*
- Thyroid Cancer, Papillary/genetics*
- Thyroid Cancer, Papillary/pathology*
- Up-Regulation
- Wnt-5a Protein/biosynthesis
- Wnt-5a Protein/genetics*
- Wnt-5a Protein/metabolism
- Xenograft Model Antitumor Assays
- Zebrafish
- PubMed
- 33578293 Full text @ Biochem. Biophys. Res. Commun.
Citation
Zhou, Q., Feng, J., Yin, S., Ma, S., Wang, J., Yi, H. (2021) LncRNA FAM230B promotes the metastasis of papillary thyroid cancer by sponging the miR-378a-3p/WNT5A axis. Biochemical and Biophysical Research Communications. 546:83-89.
Abstract
Emerging evidence indicates that the dysregulation of long non-coding RNAs (lncRNAs) plays critical roles in the progression of papillary thyroid cancer (PTC). In this study, we found consistently elevated expression levels of the lncRNA FAM230B in PTC tissues, both in newly generated RNA-seq data and in datasets from the GEO and TCGA databases. We demonstrated that the expression of FAM230B can be used for the diagnosis of PTC and is also strongly associated with lymph node metastasis. The potential biological functions of FAM230B and molecular mechanisms by which it regulates PTC progression were investigated. Functionally, FAM230B promoted the migration and invasion of PTC cells in vitro and in vivo. Mechanistically, FAM230B sponged miR-378a-3p and showed competitive binding to the 3'-UTR of WNT5A. FAM230B overexpression resulted in elevated WNT5A expression and thereby regulated the epithelial-mesenchymal transition in PTC cells. Finally, we verified that both miR-378a-3p overexpression and WNT5A silencing effectively offset the impacts of FAM230B on PTC cell migration and invasion. In conclusion, our study demonstrated the oncogenic function of the lncRNA FAM230B in PTC cells, providing a novel target for PTC diagnosis and therapy.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping