PUBLICATION
The Three-Chamber Choice Behavioral Task using Zebrafish as a Model System
- Authors
- Rowe, C.J., Crowley-Perry, M., McCarthy, E., Davidson, T.L., Connaughton, V.P.
- ID
- ZDB-PUB-210504-16
- Date
- 2021
- Source
- Journal of visualized experiments : JoVE (170): (Journal)
- Registered Authors
- Connaughton, Victoria P.
- Keywords
- none
- MeSH Terms
-
- Acclimatization
- Animals
- Behavior, Animal*
- Choice Behavior*
- Cognitive Dysfunction
- Discrimination, Psychological
- Hyperglycemia/pathology
- Models, Biological*
- Task Performance and Analysis*
- Zebrafish/physiology*
- PubMed
- 33938895 Full text @ J. Vis. Exp.
Citation
Rowe, C.J., Crowley-Perry, M., McCarthy, E., Davidson, T.L., Connaughton, V.P. (2021) The Three-Chamber Choice Behavioral Task using Zebrafish as a Model System. Journal of visualized experiments : JoVE. (170):.
Abstract
Neurodegenerative diseases are age-dependent, debilitating, and incurable. Recent reports have also correlated hyperglycemia with changes in memory and/or cognitive impairment. We have modified and developed a three-chamber choice cognitive task similar to that used with rodents for use with hyperglycemic zebrafish. The testing chamber consists of a centrally located starting chamber and two choice compartments on either side, with a shoal of conspecifics used as the reward. We provide data showing that once acquired, zebrafish remember the task at least 8 weeks later. Our data indicate that zebrafish respond robustly to this reward, and we have identified cognitive deficits in hyperglycemic fish after 4 weeks of treatment. This behavioral assay may also be applicable to other studies related to cognition and memory.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping