ZFIN ID: ZDB-PUB-210416-12
In-vivo 3D imaging of Zebrafish's intersegmental vessel development by a bi-directional light-sheet illumination microscope
Qin, X., Chen, C., Wang, L., Chen, X., Liang, Y., Jin, X., Pan, W., Liu, Z., Li, H., Yang, G.
Date: 2021
Source: Biochemical and Biophysical Research Communications   557: 8-13 (Journal)
Registered Authors: Pan, Weijun
Keywords: Light-sheet illumination microscope, Maximum intensity projection, Virtual reality, Zebrafish intersegmental vessel
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Blood Vessels/embryology*
  • Blood Vessels/growth & development
  • Embryo, Nonmammalian/blood supply*
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Imaging, Three-Dimensional/methods
  • Lighting
  • Microscopy/instrumentation*
  • Microscopy/methods
  • Neovascularization, Physiologic
  • Zebrafish/embryology*
  • Zebrafish/growth & development
PubMed: 33857842 Full text @ Biochem. Biophys. Res. Commun.
Precise quantification of vascular developments in Zebrafish requires continuous in-vivo 3D imaging. Here we employed a bi-directional light-sheet illumination microscope to characterize the development process of Zebrafish's intersegmental vessels. A Virtual Reality-based method was used to measure the lengths of intersegmental vessels (ISVs). The quantified growth rates of typical ISVs can be plotted, and unusual growth of some specific vessels was also observed.