PUBLICATION
The Novel Streptococcal Transcriptional Regulator XtgS Negatively Regulates Bacterial Virulence and Directly Represses PseP Transcription
- Authors
- Liu, G., Gao, T., Zhong, X., Ma, J., Zhang, Y., Zhang, S., Wu, Z., Pan, Z., Zhu, Y., Yao, H., Liu, Y., Lu, C.
- ID
- ZDB-PUB-210407-79
- Date
- 2020
- Source
- Infection and Immunity 88(10): (Journal)
- Registered Authors
- Keywords
- Streptococcus agalactiae, XtgS, transcriptional regulator, virulence
- MeSH Terms
-
- Animals
- Bacterial Proteins/genetics
- Bacterial Proteins/metabolism*
- Gene Expression Regulation, Bacterial
- Humans
- Phylogeny
- Promoter Regions, Genetic
- Protein Binding
- Streptococcal Infections/metabolism
- Streptococcal Infections/microbiology*
- Streptococcus/classification
- Streptococcus/genetics
- Streptococcus agalactiae/genetics
- Streptococcus agalactiae/pathogenicity*
- Transcription Factors/genetics
- Transcription Factors/metabolism*
- Transcription, Genetic
- Virulence/genetics
- Zebrafish
- PubMed
- 32690636 Full text @ Infect. Immun.
Citation
Liu, G., Gao, T., Zhong, X., Ma, J., Zhang, Y., Zhang, S., Wu, Z., Pan, Z., Zhu, Y., Yao, H., Liu, Y., Lu, C. (2020) The Novel Streptococcal Transcriptional Regulator XtgS Negatively Regulates Bacterial Virulence and Directly Represses PseP Transcription. Infection and Immunity. 88(10):.
Abstract
Streptococcus agalactiae (group B streptococcus [GBS]) has received continuous attention for its involvement in invasive infections and its broad host range. Transcriptional regulators have an important impact on bacterial adaptation to various environments. Research on transcriptional regulators will shed new light on GBS pathogenesis. In this study, we identified a novel XRE-family transcriptional regulator encoded on the GBS genome, designated XtgS. Our data demonstrate that XtgS inactivation significantly increases bacterial survival in host blood and animal challenge test, suggesting that it is a negative regulator of GBS pathogenicity. Further transcriptomic analysis and quantitative reverse transcription-PCR (qRT-PCR) mainly indicated that XtgS significantly repressed transcription of its upstream gene pseP Based on electrophoretic mobility shift and lacZ fusion assays, we found that an XtgS homodimer directly binds a palindromic sequence in the pseP promoter region. Meanwhile, the PseP and XtgS combination naturally coexists in diverse Streptococcus genomes and has a strong association with sequence type, serotype diversification and host adaptation of GBS. Therefore, this study reveals that XtgS functions as a transcriptional regulator that negatively affects GBS virulence and directly represses PseP expression, and it provides new insights into the relationships between transcriptional regulator and genome evolution.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping