PUBLICATION
Hoxd13/Bmp2-mediated mechanism involved in zebrafish finfold design
- Authors
- Castro, J., Beviano, V., Paço, A., Leitão-Castro, J., Cadete, F., Francisco, M., Freitas, R.
- ID
- ZDB-PUB-210401-4
- Date
- 2021
- Source
- Scientific Reports 11: 7165 (Journal)
- Registered Authors
- Freitas, Renata
- Keywords
- none
- MeSH Terms
-
- Animal Fins/embryology*
- Animals
- Animals, Genetically Modified
- Apoptosis/genetics
- Body Patterning
- Bone Morphogenetic Protein 2/metabolism*
- Down-Regulation
- Embryo, Nonmammalian
- Gene Expression Regulation, Developmental*
- Homeodomain Proteins/genetics*
- Homeodomain Proteins/metabolism
- Models, Animal
- Models, Biological
- Mutation
- Signal Transduction/genetics
- Skeleton/embryology
- Transcription Factors/genetics*
- Transcription Factors/metabolism
- Up-Regulation
- Zebrafish
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism*
- PubMed
- 33785799 Full text @ Sci. Rep.
Citation
Castro, J., Beviano, V., Paço, A., Leitão-Castro, J., Cadete, F., Francisco, M., Freitas, R. (2021) Hoxd13/Bmp2-mediated mechanism involved in zebrafish finfold design. Scientific Reports. 11:7165.
Abstract
The overexpression of hoxd13a during zebrafish fin development causes distal endochondral expansion and simultaneous reduction of the finfold, mimicking the major events thought to have happened during the fin-to-limb transition in Vertebrates. We investigated the effect of hoxd13a overexpression on putative downstream targets and found it to cause downregulation of proximal fin identity markers (meis1 and emx2) and upregulation of genes involved in skeletogenesis/patterning (fbn1, dacha) and AER/Finfold maintenance (bmps). We then show that bmp2b overexpression leads to finfold reduction, recapitulating the phenotype observed in hoxd13a-overexpressing fins. In addition, we show that during the development of the long finfold in leot1/lofdt1 mutants, hoxd13a and bmp2b are downregulated. Our results suggest that modulation of the transcription factor Hoxd13 during evolution may have been involved in finfold reduction through regulation of the Bmp signalling that then activated apoptotic mechanisms impairing finfold elongation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping