PUBLICATION
Ecotoxicogenomic analysis of zebrafish embryos exposed to triclosan and mixture triclosan and methyl triclosan using suppression subtractive hybridization and next-generation sequencing
- Authors
- Fu, J., Gong, Z., Bae, S.
- ID
- ZDB-PUB-210307-39
- Date
- 2021
- Source
- Journal of hazardous materials 414: 125450 (Journal)
- Registered Authors
- Gong, Zhiyuan
- Keywords
- Metabolomics, Methyl-triclosan (MTCS), Transcriptomics, Triclosan (TCS), Zebrafish embryo
- MeSH Terms
-
- Animals
- High-Throughput Nucleotide Sequencing
- Subtractive Hybridization Techniques
- Triclosan*/analogs & derivatives
- Triclosan*/toxicity
- Water Pollutants, Chemical*/toxicity
- Zebrafish/genetics
- PubMed
- 33676256 Full text @ J. Hazard. Mater.
Citation
Fu, J., Gong, Z., Bae, S. (2021) Ecotoxicogenomic analysis of zebrafish embryos exposed to triclosan and mixture triclosan and methyl triclosan using suppression subtractive hybridization and next-generation sequencing. Journal of hazardous materials. 414:125450.
Abstract
Triclosan (TCS) and methyl-triclosan (MTCS), an environmental transformation product of biocide of TCS, have been detected in water, sediment, fish, and invertebrates. In this study, the key pathway perturbation in zebrafish (Danio rerio) embryos exposed to TCS (300 μg/L) and TCS/MTCS mixture (300 μg/L TCS + 30 μg/L MTCS) was assessed by integrating the metabolomic and transcriptomic dysregulation. The differential expressed genes (DEGs) were obtained from the subtracted cDNA libraries by using the suppression subtractive hybridization and next-generation sequencing approach. The dysregulation of twenty-eight GO terms and four KEGG pathways, including oxidative phosphorylation and cardiac muscle contraction, were shown in the TCS treatment group, indicating that TCS could disrupt the mitochondrial inner membrane function by downshifting the electrochemical gradient. Meanwhile, the addition of MTCS in the exposure would cause fourteen additional significant KEGG pathway changes, demonstrating the different effects between two exposure. A pathway-based analysis using the identified DEGs and the altered metabolites in zebrafish embryos treated with TCS and TCS/MTCS mixture, collectively, has been applied. This study demonstrated that the integration of SSH-NGS and metabolomics could reveal toxic effects and potential diseases associated with the exposures of TCS and MTCS in aquatic environments.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping