PUBLICATION
Coumarins from Seseli devenyense Simonk.: Isolation by Liquid-Liquid Chromatography and Potential Anxiolytic Activity Using an In Vivo Zebrafish Larvae Model
- Authors
- Widelski, J., Luca, S.V., Skiba, A., Maciąg, M., Budzyńska, B., Marcourt, L., Wolfender, J.L., Skalicka-Woźniak, K.
- ID
- ZDB-PUB-210307-27
- Date
- 2021
- Source
- International Journal of Molecular Sciences 22(4): (Journal)
- Registered Authors
- Keywords
- Apiaceae, Danio rerio, Seseli devenyense, anxiety, central nervous system, countercurrent chromatography
- MeSH Terms
-
- Animals
- Anti-Anxiety Agents*/chemistry
- Anti-Anxiety Agents*/isolation & purification
- Anti-Anxiety Agents*/pharmacology
- Chromatography, Liquid
- Coumarins*/chemistry
- Coumarins*/isolation & purification
- Coumarins*/pharmacology
- Embryo, Nonmammalian/embryology*
- Embryonic Development/drug effects*
- Fruit/chemistry*
- Plants/chemistry*
- Zebrafish/embryology*
- PubMed
- 33673195 Full text @ Int. J. Mol. Sci.
Citation
Widelski, J., Luca, S.V., Skiba, A., Maciąg, M., Budzyńska, B., Marcourt, L., Wolfender, J.L., Skalicka-Woźniak, K. (2021) Coumarins from Seseli devenyense Simonk.: Isolation by Liquid-Liquid Chromatography and Potential Anxiolytic Activity Using an In Vivo Zebrafish Larvae Model. International Journal of Molecular Sciences. 22(4):.
Abstract
Different types of anxiety disorders have become the number one mental health issue in developed countries. The search for new, safer and effective drug-like molecules among naturally derived substances faces two difficulties: an efficient method of isolation compounds with a high-purity and high-throughput animal model for activity assay. Thus, the aim of the present study was to isolate by liquid-liquid chromatography high-purity rare coumarins from the fruits of Seseli devenyense Simonk. and evaluate their anxiolytic effect (defined as reversed thimotaxis) using a 5-days post-fertilization (dpf) Danio rerio larvae model. Liquid-liquid chromatography enabled the isolation of one simple hydroxycoumarin (devenyol) and four pyranocoumarins (cis-khellactone, d-laserpitin, isolaserpitin and octanoyllomatin). The anxiolytic effect was defined as a decrease in the time spent in the boundaries of the living space (also described as reversed thigmotaxis). Our results show that all isolated courmarins exerted a significant influence on the anxiety behavior (anxiolytic activity) in the zebrafish larvae model. According to our knowledge, this is the first report of anxiolytic activity of pyranocoumarins and devenyol.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping