PUBLICATION
Histological Analysis of Gonads in Zebrafish
- Authors
- Siegfried, K.R., Steinfeld, J.S.
- ID
- ZDB-PUB-210220-40
- Date
- 2021
- Source
- Methods in molecular biology (Clifton, N.J.) 2218: 253-263 (Chapter)
- Registered Authors
- Siegfried, Kellee, Steinfeld, Jocelyn
- Keywords
- Germ cells, Histology, Ovary, Testis
- MeSH Terms
-
- Animals
- Germ Cells/metabolism
- Gonads/metabolism*
- Sex Differentiation/physiology
- Staining and Labeling/methods*
- Zebrafish/metabolism*
- PubMed
- 33606237 Full text @ Meth. Mol. Biol.
Citation
Siegfried, K.R., Steinfeld, J.S. (2021) Histological Analysis of Gonads in Zebrafish. Methods in molecular biology (Clifton, N.J.). 2218:253-263.
Abstract
Zebrafish is an excellent system for the study of gonad development due to available genetic tools and its utilization as a human disease model. The zebrafish serves as an experimental system to model human disorders affecting the reproductive system, toxicological effects on fertility and sexual development, and hormonal regulation of fertility. Forward genetic screens have been used to uncover genetic causes of infertility and reverse genetic approaches have demonstrated that genes involved in germ cell development have similar functions in zebrafish and mammals. The most comprehensive picture of the gonad can be visualized by histology. There are a variety of methods that give excellent histology of zebrafish gonads. Below are methods for two staining approaches for the histology of paraffin-embedded zebrafish gonads.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping