ZFIN ID: ZDB-PUB-210220-37
Experimental Methodologies to Visualize Early Cell Biology in Zebrafish Embryos
Borbora, A.S., Nair, S.
Date: 2021
Source: Methods in molecular biology (Clifton, N.J.)   2218: 195-208 (Chapter)
Registered Authors: Nair, Sreelaja
Keywords: Antibody staining, Early development, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Zebrafish
MeSH Terms:
  • Animals
  • Embryo, Nonmammalian/metabolism*
  • Embryo, Nonmammalian/physiology*
  • Female
  • Immunohistochemistry/methods*
  • Male
  • Zebrafish/metabolism*
  • Zebrafish/physiology*
  • Zebrafish Proteins/metabolism
PubMed: 33606233 Full text @ Meth. Mol. Biol.
For organisms to function normally, biological molecules must work at the correct time in the right cells and within the right intracellular compartments of cells. Biological research relies heavily on discovering the cellular locations at which such molecular interactions occur. A mainstay technique in this process of discovery is the visualization of locations of proteins in cells and tissues, known as immunocytochemistry and immunohistochemistry, respectively. If performed correctly, these techniques can provide detailed information regarding the endogenous locations of proteins and their ectopic locations or absence in mutants and in disease states.