PUBLICATION

Cryopreservation of Pooled Sperm Samples

Authors
Matthews, J.L., Varga, Z.M.
ID
ZDB-PUB-210220-30
Date
2021
Source
Methods in molecular biology (Clifton, N.J.)   2218: 99-115 (Chapter)
Registered Authors
Matthews, Jennifer, Varga, Zoltan M.
Keywords
Cell density, Cell motility, Cryopreservation, In vitro fertilization, Protocol, Quality assessment, Quality control, Zebrafish (Danio rerio)
MeSH Terms
  • Animals
  • Cryopreservation/methods*
  • Cryoprotective Agents/pharmacology
  • Fertilization in Vitro/methods
  • Male
  • Semen Preservation/methods*
  • Sperm Motility/drug effects
  • Sperm Motility/physiology
  • Spermatozoa/cytology*
  • Spermatozoa/drug effects
  • Zebrafish/physiology
PubMed
33606226 Full text @ Meth. Mol. Biol.
Abstract
Cryopreservation of sperm cells is currently the most efficient tool for managing large and small collections of valuable genetic resources. Cryopreservation minimizes expenses for animal and facility maintenance such as personnel, water, power, and space. It extends the time offspring can be produced from individual organisms, reduces the need to maintain live populations, provides flexibility for planning future experiments and research projects, and can prevent catastrophic loss of irreplaceable research lines. In this chapter, we present the sperm collection, dilution, cryopreservation, thawing, and in vitro fertilization procedures used at the Zebrafish International Resource Center (ZIRC).
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes