PUBLICATION
Cryopreservation and Transplantation of Spermatogonial Stem Cells
- Authors
- Marinović, Z., Lujić, J., Li, Q., Iwasaki, Y., Urbányi, B., Yoshizaki, G., Horváth, Á.
- ID
- ZDB-PUB-210220-26
- Date
- 2021
- Source
- Methods in molecular biology (Clifton, N.J.) 2218: 37-47 (Chapter)
- Registered Authors
- Yoshizaki, Goro
- Keywords
- Biobanking, Conservation, Freezing, Microinjection, Surrogate production
- MeSH Terms
-
- Adult Germline Stem Cells/cytology*
- Adult Germline Stem Cells/drug effects
- Animals
- Cryopreservation/methods*
- Cryoprotective Agents/pharmacology
- Male
- Spermatogonia/cytology*
- Spermatogonia/drug effects
- Spermatozoa/cytology*
- Spermatozoa/drug effects
- Testis/cytology
- Testis/drug effects
- Transplantation/methods*
- Zebrafish/physiology
- PubMed
- 33606221 Full text @ Meth. Mol. Biol.
Citation
Marinović, Z., Lujić, J., Li, Q., Iwasaki, Y., Urbányi, B., Yoshizaki, G., Horváth, Á. (2021) Cryopreservation and Transplantation of Spermatogonial Stem Cells. Methods in molecular biology (Clifton, N.J.). 2218:37-47.
Abstract
Cryopreservation as a method that enables long-term storage of biological material has long been used for the conservation of valuable zebrafish genetic resources. However, currently, only spermatozoa of zebrafish can be successfully cryopreserved, while protocols for cryopreservation of eggs and embryos have not yet been fully developed. Transplantation of germline stem cells (GSCs) has risen as a favorable method that can bypass the current problem in cryopreservation of female genetic resources and can lead to reconstitution of fish species and lines through surrogate production. Here, we describe essential steps needed for the cryopreservation of spermatogonial stem cells (SSCs) and their utilization in the conservation of zebrafish genetic resources through SSC transplantation and surrogate production.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping