ZFIN ID: ZDB-PUB-210220-26
Cryopreservation and Transplantation of Spermatogonial Stem Cells
Marinović, Z., Lujić, J., Li, Q., Iwasaki, Y., Urbányi, B., Yoshizaki, G., Horváth, Á.
Date: 2021
Source: Methods in molecular biology (Clifton, N.J.)   2218: 37-47 (Chapter)
Registered Authors: Yoshizaki, Goro
Keywords: Biobanking, Conservation, Freezing, Microinjection, Surrogate production
MeSH Terms:
  • Adult Germline Stem Cells/cytology*
  • Adult Germline Stem Cells/drug effects
  • Animals
  • Cryopreservation/methods*
  • Cryoprotective Agents/pharmacology
  • Male
  • Spermatogonia/cytology*
  • Spermatogonia/drug effects
  • Spermatozoa/cytology*
  • Spermatozoa/drug effects
  • Testis/cytology
  • Testis/drug effects
  • Transplantation/methods*
  • Zebrafish/physiology
PubMed: 33606221 Full text @ Meth. Mol. Biol.
Cryopreservation as a method that enables long-term storage of biological material has long been used for the conservation of valuable zebrafish genetic resources. However, currently, only spermatozoa of zebrafish can be successfully cryopreserved, while protocols for cryopreservation of eggs and embryos have not yet been fully developed. Transplantation of germline stem cells (GSCs) has risen as a favorable method that can bypass the current problem in cryopreservation of female genetic resources and can lead to reconstitution of fish species and lines through surrogate production. Here, we describe essential steps needed for the cryopreservation of spermatogonial stem cells (SSCs) and their utilization in the conservation of zebrafish genetic resources through SSC transplantation and surrogate production.