PUBLICATION
Electron dose rate and oxygen depletion protect zebrafish embryos from radiation damage
- Authors
- Pawelke, J., Brand, M., Hans, S., Hideghéty, K., Karsch, L., Lessmann, E., Löck, S., Schürer, M., Rita Szabó, E., Beyreuther, E.
- ID
- ZDB-PUB-210216-18
- Date
- 2021
- Source
- Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology 158: 7-12 (Journal)
- Registered Authors
- Brand, Michael, Hans, Stefan
- Keywords
- Electron Flash effect, Normal tissue toxicity, Oxygen depletion, Zebrafish embryo
- MeSH Terms
-
- Animals
- Electrons*
- Oxygen
- Protons
- Zebrafish*
- PubMed
- 33587970 Full text @ Radiother Oncol
Citation
Pawelke, J., Brand, M., Hans, S., Hideghéty, K., Karsch, L., Lessmann, E., Löck, S., Schürer, M., Rita Szabó, E., Beyreuther, E. (2021) Electron dose rate and oxygen depletion protect zebrafish embryos from radiation damage. Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology. 158:7-12.
Abstract
Background and purpose In consequence of a previous study, where no protecting proton Flash effect was found for zebrafish embryos, potential reasons and requirements for inducing a Flash effect should be investigated with higher pulse dose rate and partial oxygen pressure (pO2) as relevant parameters.
Materials and methods The experiments were performed at the research electron accelerator ELBE, whose variable pulse structure enables dose delivery as electron Flash and quasi-continuously (reference irradiation). Zebrafish embryos were irradiated with ∼26 Gy either continuously at a dose rate of ∼6.7 Gy/min (reference) or by 1441 electron pulses within 111µs at a pulse dose rate of 109 Gy/s and a mean dose rate of 105 Gy/s, respectively. Using the OxyLite system to measure the pO2 a low- (pO2 ≤ 5 mmHg) and a high-pO2 group were defined on basis of the oxygen depletion kinetics in sealed embryo samples.
Results A protective Flash effect was seen for most endpoints ranging from 4 % less reduction in embryo length to about 20 - 25% less embryos with spinal curvature and pericardial edema, relative to reference irradiation. The reduction of pO2 below atmospheric levels (148 mmHg) resulted in higher protection, which was however more pronounced in the low-pO2 group.
Conclusion The Flash experiment at ELBE showed that the zebrafish embryo model is appropriate for studying the radiobiological response of high dose rate irradiation. The applied high pulse dose rate was confirmed as important beam parameter as well as the pivotal role of pO2 during irradiation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping