PUBLICATION
From local resynchronization to global pattern recovery in the zebrafish segmentation clock
- Authors
- Uriu, K., Liao, B.K., Oates, A.C., Morelli, L.G.
- ID
- ZDB-PUB-210216-16
- Date
- 2021
- Source
- eLIFE 10: (Journal)
- Registered Authors
- Oates, Andrew
- Keywords
- developmental biology, physics of living systems, zebrafish
- MeSH Terms
-
- Animals
- Biological Clocks*
- Body Patterning
- Gene Expression Regulation, Developmental
- Receptors, Notch/genetics
- Receptors, Notch/metabolism
- Signal Transduction
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/physiology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 33587039 Full text @ Elife
Citation
Uriu, K., Liao, B.K., Oates, A.C., Morelli, L.G. (2021) From local resynchronization to global pattern recovery in the zebrafish segmentation clock. eLIFE. 10:.
Abstract
Integrity of rhythmic spatial gene expression patterns in the vertebrate segmentation clock requires local synchronization between neighboring cells by Delta-Notch signaling and its inhibition causes defective segment boundaries. Whether deformation of the oscillating tissue complements local synchronization during patterning and segment formation is not understood. We combine theory and experiment to investigate this question in the zebrafish segmentation clock. We remove a Notch inhibitor, allowing resynchronization, and analyze embryonic segment recovery. We observe unexpected intermingling of normal and defective segments, and capture this with a new model combining coupled oscillators and tissue mechanics. Intermingled segments are explained in the theory by advection of persistent phase vortices of oscillators. Experimentally observed changes in recovery patterns are predicted in the theory by temporal changes in tissue length and cell advection pattern. Thus, segmental pattern recovery occurs at two length and time scales: rapid local synchronization between neighboring cells, and the slower transport of the resulting patterns across the tissue through morphogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping