PUBLICATION
Py3-FITC: a new fluorescent probe for live cell imaging of collagen-rich tissues and ionocytes
- Authors
- Wang, Z., Mizoguchi, T., Kuribara, T., Nakajima, M., Iwata, M., Sakamoto, Y., Nakamura, H., Murayama, T., Nemoto, T., Itoh, M.
- ID
- ZDB-PUB-210210-11
- Date
- 2021
- Source
- Open Biology 11: 200241 (Journal)
- Registered Authors
- Itoh, Motoyuki, Mizoguchi, Takamasa
- Keywords
- collagen-rich tissue, ionocyte, live imaging, polypyrrole, zebrafish
- MeSH Terms
-
- Animals
- Cartilage/cytology
- Cartilage/metabolism
- Cell Line
- Collagen/metabolism*
- Fluorescein-5-isothiocyanate/analogs & derivatives*
- Fluorescent Dyes/adverse effects
- Fluorescent Dyes/chemical synthesis*
- Fluorescent Dyes/pharmacokinetics
- Humans
- Microscopy, Fluorescence/methods
- Nylons/chemistry
- Polymers/chemistry
- Pyrroles/chemistry
- Zebrafish
- PubMed
- 33561382 Full text @ Open Biol.
Citation
Wang, Z., Mizoguchi, T., Kuribara, T., Nakajima, M., Iwata, M., Sakamoto, Y., Nakamura, H., Murayama, T., Nemoto, T., Itoh, M. (2021) Py3-FITC: a new fluorescent probe for live cell imaging of collagen-rich tissues and ionocytes. Open Biology. 11:200241.
Abstract
Polypyrrole-based polyamides are used as sequence-specific DNA probes. However, their cellular uptake and distribution are affected by several factors and have not been extensively studied in vivo. Here, we generated a series of fluorescence-conjugated polypyrrole compounds and examined their cellular distribution using live zebrafish and cultured human cells. Among the evaluated compounds, Py3-FITC was able to visualize collagen-rich tissues, such as the jaw cartilage, opercle and bulbus arteriosus, in early-stage living zebrafish embryos. Then, we stained cultured human cells with Py3-FITC and found that the staining became more intense as the amount of collagen was increased. In addition, Py3-FITC-stained HR cells, which represent a type of ionocyte on the body surface of living zebrafish embryos. Py3-FITC has low toxicity, and collagen-rich tissues and ionocytes can be visualized when soaked in Py3-FITC solution. Therefore, Py3-FITC may be a useful live imaging tool for detecting changes in collagen-rich tissue and ionocytes, including their mammalian analogues, during both normal development and disease progression.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping