PUBLICATION
Rbm46, a novel germ cell specific factor, modulates meiotic progression and spermatogenesis
- Authors
- Dai, X., Cheng, X., Huang, J., Gao, Y., Wang, D., Feng, Z., Zhai, G., Lou, Q., He, J., Wang, Z., Yin, Z.
- ID
- ZDB-PUB-210202-22
- Date
- 2021
- Source
- Biology of reproduction 104(5): 1139-1153 (Journal)
- Registered Authors
- He, Jiangyan, Yin, Zhan
- Keywords
- Rbm46, infertility, meiosis defects, spermatogenesis, zebrafish
- MeSH Terms
-
- Animals
- Male
- Meiosis*
- RNA-Binding Proteins/genetics*
- RNA-Binding Proteins/metabolism
- Spermatogenesis/genetics*
- Spermatogonia
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 33524105 Full text @ Biol. Reprod.
Citation
Dai, X., Cheng, X., Huang, J., Gao, Y., Wang, D., Feng, Z., Zhai, G., Lou, Q., He, J., Wang, Z., Yin, Z. (2021) Rbm46, a novel germ cell specific factor, modulates meiotic progression and spermatogenesis. Biology of reproduction. 104(5):1139-1153.
Abstract
It has been suggested that many novel RNA-binding proteins (RBPs) are required for gametogenesis, but the necessity of few of these proteins has been functionally verified. Here, we identified one member, Rbm46 and investigated its expression and role during zebrafish reproduction. We found that is maternally provided and specifically expressed in the germ cells of gonadal tissues using in situ hybridization, RT-PCR and qRT-PCR. Utilizing TALEN (transcription activator-like effector nuclease) techniques, two independent rbm46 mutant zebrafish lines were generated. Specific disruption of rbm46 resulted in masculinization and infertility in the mutants. Although the spermatogonia appeared grossly normal, spermatogenesis was impaired and meiosis events were not observed in the mutants. The introduction of a tp53M214K mutation could not rescue the female-to-male sex-reversal phenotype, which suggests that rbm46 acts independently of p53 dependent apoptotic pathway. RNA-seq and qRT-PCR results subsequently indicate that Rbm46 might be involved in post-transcriptional regulation of functional genes essential for the germ cell development such as nanos3, dazl, sycp3 during gametogenesis. Altogether, our results firstly reveal a crucial in vivo role of rbm46 in regulating germ cell development by promoting the transition from mitosis to meiosis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping