PUBLICATION
Serotonin level as a potent diabetes biomarker based on electrochemical sensing: a new approach in a zebra fish model
- Authors
- Khoshnevisan, K., Baharifar, H., Torabi, F., Sadeghi Afjeh, M., Maleki, H., Honarvarfard, E., Mohammadi, H., Sajjadi-Jazi, S.M., Mahmoudi-Kohan, S., Faridbod, F., Larijani, B., Saadat, F., Faridi Majidi, R., Khorramizadeh, M.R.
- ID
- ZDB-PUB-210128-22
- Date
- 2021
- Source
- Analytical and bioanalytical chemistry 413(6): 1615-1627 (Journal)
- Registered Authors
- Khorramizadeh, M.Reza, Mohammadi, Hassan
- Keywords
- Animal model, Biomarker, Diabetes mellitus, Electrochemical sensing, Nanocomposite, Serotonin level
- MeSH Terms
-
- Animals
- Ascorbic Acid/analysis
- Biomarkers/metabolism
- Diabetes Mellitus/diagnosis*
- Dopamine/analysis
- Electrochemical Techniques/methods*
- Electrodes
- Glucose/analysis
- Gold/chemistry
- Hydrogen Bonding
- Limit of Detection
- Metal Nanoparticles/chemistry
- Microscopy, Electron, Scanning
- Microscopy, Electron, Transmission
- Nanocomposites/chemistry
- Particle Size
- Reproducibility of Results
- Serotonin/analysis*
- Tryptophan/analysis
- Urea/analysis
- Zebrafish
- PubMed
- 33501550 Full text @ Anal. Bioanal. Chem.
Citation
Khoshnevisan, K., Baharifar, H., Torabi, F., Sadeghi Afjeh, M., Maleki, H., Honarvarfard, E., Mohammadi, H., Sajjadi-Jazi, S.M., Mahmoudi-Kohan, S., Faridbod, F., Larijani, B., Saadat, F., Faridi Majidi, R., Khorramizadeh, M.R. (2021) Serotonin level as a potent diabetes biomarker based on electrochemical sensing: a new approach in a zebra fish model. Analytical and bioanalytical chemistry. 413(6):1615-1627.
Abstract
Serotonin (5-HT) levels have been associated with several exclusively metabolic disorders. Herein, a new approach for 5-HT level as a novel biomarker of diabetes mellitus is considered using a simple nanocomposite and HPLC method. Reduced graphene oxide (rGO) comprising gold nanoparticles (AuNPs) was decorated with 18-crown-6 (18.Cr.6) to fabricate a simple nanocomposite (rGO-AuNPs-18.Cr.6). The nanocomposite was positioned on a glassy carbon electrode (GCE) to form an electrochemical sensor for the biomarker 5-HT in the presence of L-tryptophan (L-Trp), dopamine (DA), ascorbic acid (AA), urea, and glucose. The nanocomposite exhibited efficient catalytic activity for 5-HT detection by square-wave voltammetry (SWV). The proposed sensor displayed high selectivity, excellent reproducibility, notable anti-interference ability, and long-term stability even after 2 months. SWV defined a linear range of 5-HT concentration from 0.4 to 10 μg L-1. A diabetic animal model (diabetic zebrafish model) was then applied to investigate 5-HT as a novel biomarker of diabetes. A limit of detection (LOD) of about 0.33 μg L-1 was found for the diabetic group and 0.15 μg L-1 for the control group. The average levels of 5-HT obtained were 9 and 2 μg L-1 for control and diabetic groups, respectively. The recovery, relative standard deviation (RSD), and relative error (RE) were found to be about 97%, less than 2%, and around 3%, respectively. The significant reduction in 5-HT level in the diabetic group compared to the control group proved that the biomarker 5-HT can be applied for the early diagnosis of diabetes mellitus.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping