PUBLICATION
No observed effect on brain vasculature of Alzheimer's disease-related mutations in the zebrafish presenilin 1 gene
- Authors
- Barthelson, K., Newman, M., Nowell, C.J., Lardelli, M.
- ID
- ZDB-PUB-210128-2
- Date
- 2021
- Source
- Molecular brain 14: 22 (Other)
- Registered Authors
- Lardelli, Michael, Newman, Morgan
- Keywords
- 3D reconstruction, Confocal laser scanning microscopy, Vasculature, Zebrafish
- MeSH Terms
-
- Alzheimer Disease/genetics*
- Animals
- Brain/blood supply*
- Green Fluorescent Proteins/metabolism
- Heterozygote
- Mutation/genetics*
- Presenilin-1/genetics*
- Zebrafish/genetics*
- Zebrafish Proteins/genetics*
- PubMed
- 33494778 Full text @ Mol. Brain
Citation
Barthelson, K., Newman, M., Nowell, C.J., Lardelli, M. (2021) No observed effect on brain vasculature of Alzheimer's disease-related mutations in the zebrafish presenilin 1 gene. Molecular brain. 14:22.
Abstract
Previously, we found that brains of adult zebrafish heterozygous for Alzheimer's disease-related mutations in their presenilin 1 gene (psen1, orthologous to human PSEN1) show greater basal expression levels of hypoxia responsive genes relative to their wild type siblings under normoxia, suggesting hypoxic stress. In this study, we investigated whether this might be due to changes in brain vasculature. We generated and compared 3D reconstructions of GFP-labelled blood vessels of the zebrafish forebrain from heterozygous psen1 mutant zebrafish and their wild type siblings. We observed no statistically significant differences in vessel density, surface area, overall mean diameter, overall straightness, or total vessel length normalised to the volume of the telencephalon. Our findings do not support that changes in vascular morphology are responsible for the increased basal expression of hypoxia responsive genes in psen1 heterozygous mutant brains.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping