PUBLICATION

Calcineurin controls proximodistal blastema polarity in zebrafish fin regeneration

Authors

Cao, Z., Meng, Y., Gong, F., Xu, Z., Liu, F., Fang, M., Zou, L., Liao, X., Wang, X., Luo, L., Li, X., Lu, H.

ID

ZDB-PUB-210101-6

Date

2021

Source

Proceedings of the National Academy of Sciences of the United States of America 118(2): (Journal)

Registered Authors

Lu, Huiqiang, Luo, Lingfei

Keywords

calcineurin, fin regeneration, polarity, zebrafish

Datasets

GEO:GSE159560

MeSH Terms

Regeneration/physiology*
Wound Healing/physiology
Calcineurin/metabolism*
Signal Transduction
Cell Polarity/physiology
Extremities/physiology
Zebrafish Proteins
Zebrafish/metabolism
Animal Fins/physiology*
Animals

(all 10)

PubMed

33376206 Full text @ Proc. Natl. Acad. Sci. USA

Abstract

Planarian flatworms regenerate their heads and tails from anterior or posterior wounds and this regenerative blastema polarity is controlled by Wnt/β-catenin signaling. It is well known that a regeneration blastema of appendages of vertebrates such as fish and amphibians grows distally. However, it remains unclear whether a regeneration blastema in vertebrate appendages can grow proximally. Here, we show that a regeneration blastema in zebrafish fins can grow proximally along the proximodistal axis by calcineurin inhibition. We used fin excavation in adult zebrafish to observe unidirectional regeneration from the anterior cut edge (ACE) to the posterior cut edge (PCE) of the cavity and this unidirectional regeneration polarity occurs as the PCE fails to build blastemas. Furthermore, we found that calcineurin activities in the ACE were greater than in the PCE. Calcineurin inhibition induced PCE blastemas, and calcineurin hyperactivation suppressed fin regeneration. Collectively, these findings identify calcineurin as a molecular switch to specify the PCE blastema of the proximodistal axis and regeneration polarity in zebrafish fin.

Genes / Markers

Figures

Show all Figures

Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT	chemical treatment by environment: cyclosporin A, amputation: caudal fin	Adult	blastema proximal/distal pattern formation spatial distribution of a process, abnormal	Fig. 5
WT	chemical treatment by environment: cyclosporin A, amputation: caudal fin	Adult	blastema regulation of calcineurin-mediated signaling cellular spatiotemporal quality, abnormal	Fig. 5
WT	chemical treatment by environment: cyclosporin A, amputation: caudal fin	Adult	blastema regulation of calcineurin-mediated signaling decreased magnitude, abnormal	Fig. 5
WT	chemical treatment by environment: cyclosporin A, amputation: caudal fin	Adult	caudal fin regulation of direction of cell growth spatial distribution of a process, abnormal	Fig. 5
WT	chemical treatment by environment: cyclosporin A, amputation: caudal fin	Adult	regenerating fin polarity specification of proximal/distal axis cellular spatiotemporal quality, abnormal	Fig. 5
WT	amputation: caudal fin	Prim-5 to Pec-fin	blastema proximal side fkbp10b expression increased amount, abnormal	Fig. 3
WT	amputation: caudal fin	Prim-5 to Pec-fin	blastema proximal side fkbp14 expression increased amount, abnormal	Fig. 3
WT	amputation: caudal fin	Prim-5 to Pec-fin	blastema proximal side fkbp7 expression increased amount, abnormal	Fig. 3
WT	amputation: caudal fin	Prim-5 to Pec-fin	blastema proximal side fkbp9 expression increased amount, abnormal	Fig. 3
WT	amputation: caudal fin	Adult	blastema distal side ppp3ca expression increased amount, abnormal	Fig. 5

1 - 10 of 33

Show

Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
cq105		Point Mutation	cav3
dt2		Unknown	lof
pd1Tg	Tg(hsp70l:dnfgfr1a-EGFP)	Transgenic Insertion
sd2Tg	Tg(gata1a:DsRed)	Transgenic Insertion
tyt203Tg	Tg(hsp70l:mCherry-2A-fgf20a)	Transgenic Insertion
y1Tg	Tg(fli1:EGFP)	Transgenic Insertion

1 - 6 of 6

Show

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
cav3^cq105/cq105
lof^dt2/dt2
WT

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
DsRed	EFG	DsRed
EGFP	EFG	EGFP
mCherry	EFG	mCherry

Mapping

Cao et al., 2021 ZDB-PUB-210101-6 PMID:33376206

Calcineurin controls proximodistal blastema polarity in zebrafish fin regeneration

Cao et al., 2021

ZDB-PUB-210101-6
PMID:33376206