PUBLICATION
Effects of anti-inflammatory diclofenac assessed by toxicity tests and biomarkers in adults and larvae of Danio rerio
- Authors
- de Carvalho Penha, L.C., Rola, R.C., Dos Reis Martinez, C.B., de Martinez Gaspar Martins, C.
- ID
- ZDB-PUB-201219-12
- Date
- 2020
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 242: 108955 (Journal)
- Registered Authors
- Rola, Regina Coimbra
- Keywords
- Acute test, Drug, PPCP, Sublethal effects, Zebrafish
- MeSH Terms
-
- Animals
- Anti-Inflammatory Agents, Non-Steroidal/toxicity*
- Biomarkers/metabolism
- Diclofenac/toxicity*
- Lethal Dose 50
- Oxidative Stress
- Toxicity Tests
- Water Pollutants, Chemical/toxicity*
- Zebrafish/embryology
- Zebrafish/growth & development*
- Zebrafish/metabolism
- PubMed
- 33338643 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
de Carvalho Penha, L.C., Rola, R.C., Dos Reis Martinez, C.B., de Martinez Gaspar Martins, C. (2020) Effects of anti-inflammatory diclofenac assessed by toxicity tests and biomarkers in adults and larvae of Danio rerio. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 242:108955.
Abstract
The entrance of the anti-inflammatory diclofenac in water bodies is a consequence of inappropriate use, incorrect disposal, and inefficiency of wastewater treatment plants (WWTPs) in removing this drug from sewage, among others. Effects of diclofenac on non-target aquatic organisms still need to be clarified. The objective of this work was to evaluate the toxic effects of the diclofenac on larvae and adults of Danio rerio. The LC50 values were 5.49 mg/L and 5.22 mg/L for the adult and larvae, respectively. A set of biochemical and genotoxic biomarkers were evaluated in fish exposed to an environmentally relevant concentration of 2 μg/L DCF and a no observed effect concentration (NOEC) of 3 mg/L diclofenac. At the NOEC, an increase in activities of glutathione-S-transferase (GST) enzyme and an increase in ATP binding cassette (ABC) transporters in gills of adult fish was observed; also, an increase in lipoperoxidation (LPO) was seen in the gills of adults and whole larvae. These results indicate that diclofenac activates the fish detoxification processes and may affect fish health.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping