ZFIN ID: ZDB-PUB-201217-11
Using Zebrafish Larvae as a Xenotransplantation Model to Study Ewing Sarcoma
Pascoal, S., Grissenberger, S., Scheuringer, E., Fior, R., Ferreira, M.G., Distel, M.
Date: 2021
Source: Methods in molecular biology (Clifton, N.J.)   2226: 243-255 (Chapter)
Registered Authors: Distel, Martin, Ferreira, Miguel Godinho, Fior, Rita, Grissenberger, Sarah, Pascoal, Susana, Scheuringer, Eva
Keywords: Ewing sarcoma, Live imaging, Metastasis, Tumor model, Xenotransplantation, Zebrafish
MeSH Terms:
  • Animals
  • Biomarkers
  • Bone Neoplasms/pathology*
  • Cell Line, Tumor
  • Disease Models, Animal*
  • Immunohistochemistry
  • Larva
  • Sarcoma, Ewing/pathology*
  • Transplantation, Heterologous*
  • Zebrafish
PubMed: 33326107 Full text @ Meth. Mol. Biol.
Tumor models allowing for the in vivo investigation of molecular mechanisms driving tumor progression and metastasis are important to develop novel strategies for cancer treatment. Unfortunately, for Ewing sarcoma no adequate genetic animal models are currently available. Mouse xenograft models are the state of the art to model Ewing sarcoma in vivo. Here, we describe an alternative Ewing sarcoma xenograft model in embryonic and larval zebrafish. This xenograft model offers live imaging and easy compound testing opportunities hereby complementing mouse xenograft models. In this chapter, we provide a detailed protocol how to xenograft Ewing sarcoma cells (shSK-E17T) into 2-day-old zebrafish and how xenografted zebrafish can be imaged and analyzed over consecutive days to study tumor proliferation.