PUBLICATION
Using Zebrafish Larvae as a Xenotransplantation Model to Study Ewing Sarcoma
- Authors
- Pascoal, S., Grissenberger, S., Scheuringer, E., Fior, R., Ferreira, M.G., Distel, M.
- ID
- ZDB-PUB-201217-11
- Date
- 2021
- Source
- Methods in molecular biology (Clifton, N.J.) 2226: 243-255 (Chapter)
- Registered Authors
- Distel, Martin, Ferreira, Miguel Godinho, Fior, Rita, Grissenberger, Sarah, Pascoal, Susana, Scheuringer, Eva
- Keywords
- Ewing sarcoma, Live imaging, Metastasis, Tumor model, Xenotransplantation, Zebrafish
- MeSH Terms
-
- Animals
- Biomarkers
- Bone Neoplasms/pathology*
- Cell Line, Tumor
- Disease Models, Animal*
- Immunohistochemistry
- Larva
- Sarcoma, Ewing/pathology*
- Transplantation, Heterologous*
- Zebrafish
- PubMed
- 33326107 Full text @ Meth. Mol. Biol.
Citation
Pascoal, S., Grissenberger, S., Scheuringer, E., Fior, R., Ferreira, M.G., Distel, M. (2021) Using Zebrafish Larvae as a Xenotransplantation Model to Study Ewing Sarcoma. Methods in molecular biology (Clifton, N.J.). 2226:243-255.
Abstract
Tumor models allowing for the in vivo investigation of molecular mechanisms driving tumor progression and metastasis are important to develop novel strategies for cancer treatment. Unfortunately, for Ewing sarcoma no adequate genetic animal models are currently available. Mouse xenograft models are the state of the art to model Ewing sarcoma in vivo. Here, we describe an alternative Ewing sarcoma xenograft model in embryonic and larval zebrafish. This xenograft model offers live imaging and easy compound testing opportunities hereby complementing mouse xenograft models. In this chapter, we provide a detailed protocol how to xenograft Ewing sarcoma cells (shSK-E17T) into 2-day-old zebrafish and how xenografted zebrafish can be imaged and analyzed over consecutive days to study tumor proliferation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping