PUBLICATION
The role of miR-92 in regulating early development and metamorphosis of Japanese flounder Paralichthys olivaceus
- Authors
- Li, X., Hu, H., Li, R., Wang, Z., Qi, J., Wang, Z.
- ID
- ZDB-PUB-201215-23
- Date
- 2020
- Source
- Genes & genetic systems 95: 1-10 (Journal)
- Registered Authors
- Qi, Jie
- Keywords
- Paralichthys olivaceus, embryonic development, metamorphic development, miR-92
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Endoderm/growth & development
- Female
- Flounder/genetics*
- Flounder/growth & development
- GATA5 Transcription Factor/genetics
- GATA5 Transcription Factor/metabolism
- Gene Expression Regulation, Developmental/genetics*
- Genes, Reporter
- Japan
- Male
- Mesoderm/growth & development
- Metamorphosis, Biological
- MicroRNAs/genetics*
- Zebrafish
- PubMed
- 31839648 Full text @ Genes Genet. Sys.
Citation
Li, X., Hu, H., Li, R., Wang, Z., Qi, J., Wang, Z. (2020) The role of miR-92 in regulating early development and metamorphosis of Japanese flounder Paralichthys olivaceus. Genes & genetic systems. 95:1-10.
Abstract
MicroRNAs are a class of short non-coding RNAs that contain approximately 22 nucleotides and play a regulatory role in RNA silencing and translational repression. miR-92 belongs to the miR-17-92 family and has a regulatory effect on cell proliferation, apoptosis, and expression of proto-oncogenes and tumor suppressor genes. However, its function in flatfish is unclear. In this study, we used farmed Japanese flounder, Paralichthys olivaceus, and showed that gata5 is a target gene of miR-92. Experiments on miR-92 overexpression indicated that gata5 and sox17 were downregulated, while the transcription level of ntl increased. By contrast, depletion of miR-92 resulted in increased gata5 and sox17 levels and reduced ntl level. Moreover, thiourea treatment indicated that miR-92 may inhibit the metamorphic development of Japanese flounder. Our study suggests that miR-92 regulates the fate of endoderm and mesoderm by controlling gata5.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping