PUBLICATION
Structural elucidation of an immunological arabinan from the rhizomes of Ligusticum chuanxiong, a traditional Chinese medicine
- Authors
- Zhang, S., An, L., Li, Z., Wang, X., Wang, H., Shi, L., Bao, J., Lan, X., Zhang, E., Lall, N., Reid, A.M., Li, Y., Jin, D.Q., Xu, J., Guo, Y.
- ID
- ZDB-PUB-201215-13
- Date
- 2020
- Source
- International journal of biological macromolecules 170: 42-52 (Journal)
- Registered Authors
- Li, Yuhao
- Keywords
- Immunomodulation, Ligusticum chuanxiong, Polysaccharides
- MeSH Terms
-
- Animals
- Carbohydrate Conformation
- Carbohydrate Sequence
- Chemistry Techniques, Analytical
- Drugs, Chinese Herbal/chemistry*
- Drugs, Chinese Herbal/pharmacology
- Interleukin-1beta/metabolism
- Interleukin-6/metabolism
- Ligusticum/chemistry*
- Macrophage Activation/drug effects
- Mice
- Microscopy, Electron, Scanning
- Molecular Structure
- Molecular Weight
- Nitric Oxide/metabolism
- Nuclear Magnetic Resonance, Biomolecular
- Phagocytosis/drug effects
- Polysaccharides/chemistry*
- Polysaccharides/isolation & purification
- Polysaccharides/pharmacology
- RAW 264.7 Cells
- Reactive Oxygen Species/metabolism
- Rhizome/chemistry
- Tumor Necrosis Factor-alpha/metabolism
- Zebrafish/embryology
- Zebrafish/immunology
- PubMed
- 33316344 Full text @ Int. J. Biol. Macromol.
Citation
Zhang, S., An, L., Li, Z., Wang, X., Wang, H., Shi, L., Bao, J., Lan, X., Zhang, E., Lall, N., Reid, A.M., Li, Y., Jin, D.Q., Xu, J., Guo, Y. (2020) Structural elucidation of an immunological arabinan from the rhizomes of Ligusticum chuanxiong, a traditional Chinese medicine. International journal of biological macromolecules. 170:42-52.
Abstract
In the present study, an immunological arabinan, LCP70-2A, was isolated from Ligusticum chuanxiong for the first time. The absolute molecular weight of LCP70-2A was determined to be 6.46 × 104 g/mol using the HPSEC-MALLS-RID method. The absolute configuration of arabinose in LCP70-2A was determined to be L-configuration. Physicochemical characterization revealed that LCP70-2A was a homogeneous polysaccharide with a backbone of (1 → 5)-linked α-L-Araf with ramification at O-2 and O-3 of terminal α-L-arabinose. Molecular conformation analysis showed that LCP70-2A was a branching polysaccharide with a compact coil chain conformation in 0.1 M NaCl solution. In addition, in vitro cell assays showed that LCP70-2A can activate macrophages by enhancing the phagocytosis and potentiating the secretion of immunoregulatory factors including NO, TNF-α, IL-6, and IL-1β. Furthermore, LCP70-2A was found to promote the release of ROS and NO using the zebrafish model, suggesting that LCP70-2A can be further developed as a candidate supplement for immunological enhancement.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping