PUBLICATION
Sound generation in zebrafish with Bio-Opto-Acoustics
- Authors
- Favre-Bulle, I.A., Taylor, M.A., Marquez-Legorreta, E., Vanwalleghem, G., Poulsen, R.E., Rubinsztein-Dunlop, H., Scott, E.K.
- ID
- ZDB-PUB-201202-15
- Date
- 2020
- Source
- Nature communications 11: 6120 (Journal)
- Registered Authors
- Scott, Ethan
- Keywords
- none
- MeSH Terms
-
- Acoustic Stimulation/methods
- Acoustics*
- Animals
- Brain
- Hearing/physiology
- Larva/physiology
- Neurons
- Otolithic Membrane/physiology*
- Sound*
- Vibration
- Zebrafish/physiology*
- PubMed
- 33257652 Full text @ Nat. Commun.
Citation
Favre-Bulle, I.A., Taylor, M.A., Marquez-Legorreta, E., Vanwalleghem, G., Poulsen, R.E., Rubinsztein-Dunlop, H., Scott, E.K. (2020) Sound generation in zebrafish with Bio-Opto-Acoustics. Nature communications. 11:6120.
Abstract
Hearing is a crucial sense in underwater environments for communication, hunting, attracting mates, and detecting predators. However, the tools currently used to study hearing are limited, as they cannot controllably stimulate specific parts of the auditory system. To date, the contributions of hearing organs have been identified through lesion experiments that inactivate an organ, making it difficult to gauge the specific stimuli to which each organ is sensitive, or the ways in which inputs from multiple organs are combined during perception. Here, we introduce Bio-Opto-Acoustic (BOA) stimulation, using optical forces to generate localized vibrations in vivo, and demonstrate stimulation of the auditory system of zebrafish larvae with precise control. We use a rapidly oscillated optical trap to generate vibrations in individual otolith organs that are perceived as sound, while adjacent otoliths are either left unstimulated or similarly stimulated with a second optical laser trap. The resulting brain-wide neural activity is characterized using fluorescent calcium indicators, thus linking each otolith organ to its individual neuronal network in a way that would be impossible using traditional sound delivery methods. The results reveal integration and cooperation of the utricular and saccular otoliths, which were previously described as having separate biological functions, during hearing.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping