PUBLICATION
Effects of nanocapsules of poly-ε-caprolactone containing artemisinin on zebrafish early-life stages and adults
- Authors
- Charlie-Silva, I., Feitosa, N.M., Fukushima, H.C.S., Borra, R.C., Foglio, M.A., Xavier, R.M.P., de Melo Hoyos, D.C., de Oliveira Sousa, I.M., de Souza, G.G., Bailone, R.L., de Andrade Belo, M.A., Correia, S.A.M., Junior, J.D.C., Pierezan, F., Malafaia, G.
- ID
- ZDB-PUB-201202-11
- Date
- 2020
- Source
- The Science of the total environment 756: 143851 (Journal)
- Registered Authors
- Feitosa, Natalia Martins
- Keywords
- Danio rerio, Ecotoxicity, Embryotoxicity, Nanoparticles
- MeSH Terms
-
- Adult
- Animals
- Artemisinins*/toxicity
- Caproates
- Embryo, Nonmammalian
- Humans
- Lactones
- Nanocapsules*
- Water Pollutants, Chemical*/toxicity
- Zebrafish
- PubMed
- 33257061 Full text @ Sci. Total Environ.
Citation
Charlie-Silva, I., Feitosa, N.M., Fukushima, H.C.S., Borra, R.C., Foglio, M.A., Xavier, R.M.P., de Melo Hoyos, D.C., de Oliveira Sousa, I.M., de Souza, G.G., Bailone, R.L., de Andrade Belo, M.A., Correia, S.A.M., Junior, J.D.C., Pierezan, F., Malafaia, G. (2020) Effects of nanocapsules of poly-ε-caprolactone containing artemisinin on zebrafish early-life stages and adults. The Science of the total environment. 756:143851.
Abstract
Artemisinin extracted from Artemisia annua L. plants has a range of properties that qualifies it to treat several diseases, such as malaria and cancer. However, it has short half-life, which requires making continuous use of it, which has motivated the association of artemisinin (ART) with polymeric nanoparticles to increase its therapeutic efficiency. However, the ecotoxicological safety of this association has been questioned, given the scarcity of studies in this area. Thus, in this work the toxicity of Poly (ε-Caprolactone) nanocapsules added with ART (ART-NANO) in zebrafish (Danio rerio), embryos and adults was studied. Different endpoints were analyzed in organisms exposed to ART-NANO, including those predictive of embryotoxicity and histopatoxicity. Embryotoxicity was analyzed based on Organization for Economic Co-operation and Development (OECD) test guideline (236) for fish embryo acute toxicity applied to zebrafish (Danio rerio) at 96 hpf under five nominal logarithmic concentrations (0.125 to 2.0 mg/ L). Our results demonstrate, mainly, that fertilized eggs presented increased coagulation, lack of heart rate, vitelline sac displacement and lack of somite formation. On the other hand, adult individuals (exposed to the same concentrations and evaluated after 24 and 96 h of exposure) have shown increased pericarditis. Therefore, the treatment based on ART, poly (ε-caprolactone) nanocapsules and on their combination at different concentrations have shown toxic effects on zebrafish embryos and adult individuals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping